不同地理株嗜人按蚊对间日疟原虫的易感性  

Susceptibility of Anopheles anthropophagus from different areas of China to Plasmodium vivax

作　　者：周华云[1] 朱国鼎[1] 李菊林[1] 金小林[1] 陆凤[1] 刘耀宝[1] Jetsumon Sattabongkot 高琪[1] 

机构地区：[1]江苏省寄生虫病防治研究所、卫生部寄生虫病预防与控制技术重点实验室、江苏省寄生虫分子生物学重点实验室、江苏省寄生虫病学重点学科,无锡214064 [2]Armed ForcesResearch Institute of Medical Sciences, Thailand

出　　处：《中国血吸虫病防治杂志》2008年第4期265-268,共4页Chinese Journal of Schistosomiasis Control

基　　金：美国政府基金(WRAIR1126);“十五”国家科技攻关项目(2004BA718B13)

摘　　要：目的比较我国不同地理株嗜人按蚊对间日疟原虫的易感性。方法在我国间日疟流行区采集间日疟病例血样,采用体外人工膜饲感染系统在实验室同时体外人工感染广东、辽宁、江苏3个不同地理株的嗜人按蚊,在感染后的7-9d和14d分别解剖蚊胃和唾液腺,并检测蚊体内的卵囊和子孢子数。结果共配对感染江苏、广东和辽宁地理株嗜人按蚊35批,感染7-9d 3种按蚊卵囊阳性率分别为68.57%、60.00%和68.57%,感染后14d子孢子阳性率分别为22.86%、14.29%和22.86%,3种按蚊感染卵囊和子孢子差异无统计学意义（P〉0.05）。在感染后7-9d分别解剖江苏、辽宁、广东株嗜人按蚊228、235只和228只,卵囊阳性蚊比例分别为28.07%、25.11%和26.75%。在感染后14d分别解剖江苏、辽宁、广东株嗜人按蚊150、142只和135只,子孢子阳性蚊比例分别为10.67%、8.45%和11.85%,三者间卵囊和子孢子阳性蚊比例差异均无统计学意义（P均〉0.05）。江苏、广东、辽宁株嗜人按蚊子孢子感染度差异无统计学意义（P〉0.05）。结论广东、辽宁、江苏3个不同地理株嗜人按蚊均对间日疟原虫易感。Objective To compare the susceptibility of Anopheles anthropophagus from Jiangsu, Guangdong and Liaoning provinces in China to Plasmodium vivax.Methods The blood samples of patients with P. vivax in endemic areas of China were collected to feed the mosquitoes of An. anthropophagus from different areas by using the artificial in vitro membrane feeding system in the lab, and then the mosquitoes were dissected during the 7-9th day and on the 14th day after the feeding and the oocysts and sporozoites in the stomach and salivy gland of mosquitoes were counted. Results The mosquitoes from Jiangsu, Guangdong and Liaoning were simultaneously fed with the blood of 35 cases of P. vivax. The oocyst positive rates of An. anthropophagus from Jiangsu, Guangdong and Liaoning during the 7-9th day after the feeding were 68.57%, 60.00% and 68.57%, as well as the sporozoite positive rates of them on the 14th day after the feeding were 22.86%, 14.29% and 22.86%, respectively. On the 7th day after the feeding, 228, 235, 228 mosquitoes of An. anthropophagus from Jiangsu, Guangdong and Liaoning were dissected, and the positive mosquito rates with oocyst infection were 28.07%, 25.11% and 26.75%, respectively. On the 14th day after the feeding, 150, 142, 135 mosquitoes of An. anthropophagus from the three areas were dissected, the positive rates with sporozoite infection were 10.67%, 8.45% and 11.85%, respectively. The num-bers of mosquitoes dissected with infective grade（＂＋＂,＂＋＋＂,＂＋＋＋＂,＂＋＋＋＋＂） of sporozoites of An. anthropophagus from Jiangsu, Guangdong and Liaoning were 4, 3, 2, 7, 2, 2, 3, 7 and 1, 6, 3, 8, respectively. Conclusions An. anthropophagus from Jiangsu, Guangdong and Liaoning is susceptible to the parasites of Plasmodium vivax and there is no significant difference among the susceptibilities of An. anthropophagus from the three areas to Plasmodium vivax.

关 键 词：间日疟原虫 嗜人按蚊 人工感染 江苏省 广东省 辽宁省 

分 类 号：R382.31[医药卫生—医学寄生虫学]