血小板因子4对环磷酰胺损伤小鼠骨髓细胞增殖能力的影响  

Effects of PF4 on bone marrow cell proliferative activity in mice with CTX-induced injury

作　　者：宋俊玲[1] 杨岚[2] 田琼[3] 

机构地区：[1]武警山西总队医院内一科,太原030006 [2]第四军医大学附属西京医院血液科 [3]第四军医大学基础部药理系

出　　处：《山西医科大学学报》2008年第9期778-781,共4页Journal of Shanxi Medical University

基　　金：陕西省科技攻关基金资助项目(2006K09-G2)

摘　　要：目的探讨血小板因子4(PF4)对环磷酰胺(CTX)诱导的化学损伤小鼠骨髓细胞增殖能力的影响。方法36只小鼠随机分为PF4组、CTX组和对照组。小鼠腹腔注射CTX 200 mg/kg制备化学药物损伤小鼠模型。在注射CTX前26 h和20 h,PF4组和CTX组各注射PF4(40μg/kg)和等体积的PBS缓冲液,对照组只注射等体积的PBS缓冲液。通过体外双层琼脂培养法测定PF4对CTX损伤小鼠骨髓细胞粒-巨噬细胞集落(CFU-GM)形成的影响,流式细胞仪测定细胞周期的变化。结果注射CTX后第5天CTX组骨髓细胞数(BMK)较对照组显著减少(P<0.01);第8天PF4组BMK较CTX组显著增多(P<0.01)。注射CTX后第5天CTX组骨髓CFU-GM数量较对照组和PF4组显著减少(P<0.01);第8天PF4组与CTX组的骨髓CFU-GM数量均有不同程度的上升,但两组间差异仍有统计学意义(P<0.05)。注射CTX后第5天PF4组骨髓有核细胞G0/G1期细胞百分率比CTX组显著提高,且细胞坏死、凋亡率显著下降(P<0.01),而与对照组相比差异无统计学意义(P>0.05)。第8天各组间G0/G1期细胞百分率、细胞坏死/凋亡率差异均无统计学意义(P>0.05)。结论PF4使处于G0/G1期的骨髓细胞数增多,降低了其坏死和凋亡率,增加骨髓细胞CFU-GM集落形成率。Objective To explore the effects of PF4 on bone marrow cell proliferation in mice with CTX-induced injury. Methods Thirty-six BALB mice were randomly divided into PF4 group（ n = 12）, CTX group（ n = 12）and control group（ n = 12）. The mice were intraperitoneally injected with CTX 200 mg/kg to establish chemical lesion models. At hour 26 and 20 before administration of CTX,PF4（40μ/kg） was injected in PF4 group,and PBS in CTX group. In control group, PBS was injected without CTX. The effects of PF4 on CFU-GM forming of CTX-treated mice were evaluated. CFU-GM was assayed using a double-layer agar culture system in vitro. The DNA contents in bone marrow cells were detected by flow eytometry（FCM）. Results At day 5 after injecting CTX, the numbers of bone marrow cells in CTX group significantly decreased compared with control group（P 〈 0.01 ）. At day 8, the numbers of bone marrow cells in PF4 groups were significantly higher than that in CTX group（P 〈 0.01 ）. At day 5, bone marrow CFU- GM yields in CTX group significantly decreased compared with control group and PF4 group（P 〈 0.01）. At day 8, CFU-GM yields in PF4 group and CTX group increased significantly, higher in PF4 group than in CTX group. At day 5,bone marrow cells in G0/G1 phase in PF4 group were significantly higher than that in CTX group, and the necrotic and apoptotic percentages were significantly lower（ P 〈 0.01）, but there was no significant difference between PF4 group and control group. At day 8, there was no significant difference among three groups（P 〉0.05）. Conclusion PF4 can increase bone marrow cells in G0/G1 phase, decrease the necrotic and apoptotic percentage, and increase bone marrow CFU-GM yields.

关 键 词：血小板因子4 环磷酰胺 造血干细胞 

分 类 号：R551.3[医药卫生—血液循环系统疾病]