急性冠状动脉综合征患者IL-18/IL-10比值与Th1/Th2失衡  

Interleukin-18/interleukin-10 ratio and Th1/Th2 imbalance in patients with acute coronary syndrome

作　　者：江华[1] 李艳 刘文卫[1] 刘永胜[1] 

机构地区：[1]湖北省襄樊市中心医院,湖北襄樊441021 [2]湖北省武汉人民医院,湖北武汉430000

出　　处：《检验医学》2008年第5期466-468,共3页Laboratory Medicine

摘　　要：目的研究免疫炎性因子在急性冠状动脉综合征(ACS)中的作用机制。方法入选经冠状动脉造影确诊的ACS患者189例,26名正常人作对照。入院时即采血,并随防观察有无心脏病死亡、心绞痛再发、再梗死、心律不齐等发生。采用流式细胞术检测辅助性T细胞(Th)1与Th2,酶联免疫吸附试验(ELISA)检测白细胞介素(IL)-18和IL-10表达。结果ACS患者Th1、IL-18较正常人明显增加(P<0.01,P<0.05),而Th2和IL-10明显减少(P均<0.05),Th1/Th2与IL-18/IL-10呈正相关(r=0.532,P<0.01)。结论ACS患者Th1/Th2和IL-18/IL-10比值升高,提示ACS患者动脉斑块形成与Th1细胞及其分泌的前炎性因子IL-18升高有关。Objective To explore the mechanisms of inflammatory cytokines in acute coronary syndrome （ACS）. Methods The study groups included 189 patients with ACS diagnosed by coronary arteriography and 26 healthy controls. Blood samples were taken at their admission to the hospital. The composite of cardiac death, recurrence of unstable angina, reinfarction, arrhythmias during hospitalization was the pre-specified study end-point. Peripheral blood lymphocyte subgroups helper T cell （Th）1/Th2 were quantified by flow cytometry. Serum interleukin （IL）-10 and IL-18 levels were measured by enzyme-linked immunosorbent assay （ELISA）. Results The levels of Thl and IL-18 of patients with ACS were significantly higher than that of controls （P〈0.01,P〈0.05）, while the levels of Th2 and IL-10 reduced in the ACS group（P〈0.05,P〈0.05）. The frequency of Th1/Th2 positively correlated with the ratio of serum IL-18/IL- 10 in patients with ACS（r=0.532 ,P〈0.01）. Conclusions The frequency of Th1/Th2 and the ratio of IL-18/IL-10 increase in the patients with ACS, which shows that the formation of artery plaque in patients with ACS correlates with the high levels of Thl and pro-inflammatory cytokine IL-18.

关 键 词：白细胞介素-18 白细胞介素-10 辅助性T细胞 急性冠状动脉综合征 炎症 

分 类 号：R446.62[医药卫生—诊断学]