HPLC法测定天山花楸中芦丁的含量  

Determination of Rutin in Sorbus Tianschanica by High Performance Liquid Chromatography

作　　者：李乐[1] 李鹏[1] 张芹[1] 唐辉[1] 

机构地区：[1]新疆特种植物药资源教育部重点实验室石河子大学药学院,新疆石河子832002

出　　处：《四川中医》2008年第9期51-53,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：新疆生产建设兵团科技攻关计划课题(2006GG52)。

摘　　要：目的：建立HPLC梯度洗脱法测定天山花楸叶中芦丁含量的方法。方法：采用ZORBAX SB-C18（4.6mm×15mm,5μm）色谱柱,甲醇-0.02%磷酸溶液为流动相（梯度洗脱）,流速为1.0ml·min^-1,检测波长为326nm。结果：芦丁线性范围为0.2104-1.8936μg/ml,叶中芦丁含量0.4829mg/100ml（RSD%=0.48%,n=3）,平均回收率为100.4%。结论：该方法经济、简便、快速、准确、可靠,可用于该药材的质量控制。Objecthe： To establish the method of Rutin determination in Sorbus Tiansehaniea. Methods： ZORBAX SB - C18 （4.6mm ×15mm, 5μm） column of High Performance Liquid Chromatography （ HPLC ） was adopted in the Rutin determination. The MeOH-0.02% H3PO3 was used as mobile phase. The flow rate was 1.0ml·min^-1. The detective wavelength was 326nm. Results： The linear range of Rutin was 0.2104 - 1.893μg/ml. Rutin in the leaf of S. Tianschanica was 0.4829mg/100ml （RSD% = 0.48%, n = 3）, and the average recovery- rate was 100.4%. Conclusion： The method is simple and sensifive, and can determine Rutin content quickly and correctly. It is suitable for the analysis of Rutin in S. Tiansehanica.

关 键 词：天山花楸 芦丁 HPLC 梯度洗脱 

分 类 号：R284.1[医药卫生—中药学]