HPLC法测定感冒退热颗粒中腺苷的含量  

Determination of Adenosine in Ganmao Tuire Granules by HPLC

作　　者：赵成[1] 

机构地区：[1]安徽省六安市药品检验所,安徽六安237000

出　　处：《现代中药研究与实践》2008年第4期28-29,共2页Research and Practice on Chinese Medicines

摘　　要：目的建立HPLC法测定感冒退热颗粒中腺苷的含量，以有效控制其内在质量。方法采用VP-ODS柱（150mm×4．6mm，5μm）；磷酸盐缓冲液（pH6．5）-甲醇（17：3），检测波长260mm，流速1．0mL·min^-1，柱温为室温。结果腺苷在3．93-39．28mg·L^-1（r=0．9999，n=6）范围内线性关系良好，平均回收率为99．3％，RSD=0．61％。结论本法简便、灵敏、重复性好、准确可靠，可用于感冒退热颗粒中腺苷的测定。Objective To establish a method for the determination of adenosine in Ganmao Tuire granules. Methods The samples were separated by VP-ODS column（150mm×4.6mm,5μm） ,the mobile phase was phosphate acid buffer solution（pH 6.5 ）-methanol（ 17：3 ） ,the detection wavelength was at 260 nm ,flow rate was 1.0 mL·min^-1, the column temperature was indoor temperature. Results The linear range of adenosine was in 3.93-39.28 mg·L^- 1（r = 0.9999, n = 6 ）. The average recovery was 99.3 % with RSD of 0.61%. Conclusion This method is simple, sensitive , accurate and can be used to determine the contents of adenosine in Ganmao Tuire gralunes.

关 键 词：感冒退热颗粒 HPLC法 腺苷 含量测定 

分 类 号：R927.2[医药卫生—药学]