人类免疫缺陷病毒感染者重叠输血传播病毒感染的临床研究  

The clinical research on patients with superinfection of HIV-1 and TTV

作　　者：欧强[1] 周坚[1] 

机构地区：[1]复旦大学附属公共卫生临床中心,上海201508

出　　处：《肝脏》2008年第4期292-294,共3页Chinese Hepatology

基　　金：复旦大学上海医学院青年科研基金资助项目

摘　　要：目的了解中国地区人类免疫缺陷病毒(HIV-1)感染者重叠输血传播病毒(TTV)感染的流行情况,并探讨重叠感染TTV对CD4+T淋巴细胞计数及HIV RNA病毒载量的影响。方法收集55例HIV-1感染者的血浆标本,采用荧光定量PCR法检测TTV DNA,流式细胞法检测T淋巴细胞亚群,bDNA方法检测HIV RNA。结果HIV感染者中TTV DNA检出率及TTV DNA滴度明显高于健康对照组(P<0.05)。HIV/TTV重叠感染者CD4+T淋巴细胞计数低于单纯HIV感染者,HIV RNA病毒载量高于单纯HIV感染者,两组比较差异有统计学意义(P<0.05)。结论中国地区HIV感染者易重叠感染TTV,且滴度较高。重叠感染TTV可能导致细胞免疫功能进一步下降,加快病毒复制,促进HIV病程的进展。Objective To investigate the prevalence of superinfection of HIV-1 and TTV in China and discuss the influence of TTV co-infection in the count of CD4^＋ T cell and the load of HIV RNA. Methods The plasm of 55 cases of HIV/AIDS patients was collected. TTV DNA was detected by real time quantitative PCR. T lymphocyte cell subsets were detected by flow cytometry. HIV RNA was detected by bDNA. Results The positive rate of TTV and the titer of TTV DNA in HIV/AIDS patients were much higher than those in normal control group. There was significantly statistic difference between two groups（P〈0.05）. The count of CD4^＋ T cell count in patients with HIV/TTV co-infection was much lower than the patients with HIV infection（P〈0. （15） ,but the load of HIV RNA in patients with HIV/TTV co-infection was much higher than the patients with HIV infection（P〈0.05）. Conclusion Patients with HIV-1 infection were apt to be infected by TTV,and the titer of TTV DNA was high by contrast with the normal. TTV co-infection may damage the cell immune functioned and improve the duplication of HIV.

关 键 词：人类免疫缺陷病毒 输血传播病毒 临床研究 

分 类 号：R512.91[医药卫生—内科学] R512.6[医药卫生—临床医学]