葛根素注射液对自发性高血压大鼠AT1和ACE2 mRNA表达的影响  

Effect of Puerarin Injection on the mRNA Expressions of AT1 and ACE2 in Spontaneous Hypertension Rats

作　　者：叶绪英[1] 宋卉 卢成志[3] 

机构地区：[1]汕头大学医学院,广东515041 [2]广东省食品药品职业学院 [3]天津市第一中心医院心内科

出　　处：《中国中西医结合杂志》2008年第9期824-827,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广东省中医药管理局基金资助项目(No.1050138)

摘　　要：目的探讨葛根素对自发性高血压大鼠Ⅰ型血管紧张素Ⅱ受体(AT1)及血管紧张素转换酶2 (ACE2)的作用。方法将12周龄自发性高血压大鼠分成4组:模型对照组、维拉帕米组、低剂量葛根素组及高剂量葛根素组,给药3周后,取心肌、主动脉、肾脏组织提RNA。采用RT-PCR技术检测AT1和ACE2的mRNA表达水平。结果在心肌中,低剂量葛根素组的AT1 mRNA和ACE2 mRNA表达较模型对照组降低(P<0.05),而在肾脏组织,高剂量葛根素组的AT1 mRNA和ACE2 mRNA表达较模型对照组升高(P<0.05),ACE2的表达较低剂量葛根素组升高(P<0.05);在主动脉中,各组间比较差异均无统计学意义。在心肌和肾脏组织中,AT1 mRNA与ACE2 mRNA的表达水平呈正相关线性关系。结论高剂量葛根素升高肾脏组织中AT1和ACE2 mRNA表达,而低剂量葛根素可降低心肌中AT1和ACE2 mRNA表达,;AT1与ACE2之间可能存在正反馈调节机制。Objective To study the effect of puerarin on angiotensin Ⅱ type 1 receptor （AT1） and angiotensin-converting enzyme 2 （ACE2） in spontaneous hypertension rat （SHR）. Methods SHRs, 12 weeks old, were randomly divided into four groups： the model control group （A） , the Verapamil group （B） , and the two puerarin groups （ C and D） treated by low dose and high dose of puerarin respectively. After being treated for 3 weeks, total RNA from tissues of heart, aorta and kidney in rats were extracted and mRNA expression levels of AT1 and ACE2 were determined by RT-PCR. Results As compared with Group A, the mRNA expressions of AT1 and ACE2 in heart tissue were lower in Group C, and those in kidney tissue were higher in Group D （ all P 〈 0.05） ; ACE2 mRNA expression was higher in Group D than in Group C （ P 〈 0.05 ） ; no significant differences of the two indexes in aorta were shown among various groups. Besides, mRNA expressions of AT1 and ACE2 in heart and kidney tissue were proved to be positively linearly correlated. Conclusion High dose puerarin could increase the mRNA expressions of AT1 and ACE2 in kidney, while low dose puerarin could decrease them in heart; there might be a feed back correlation between AT1 and ACE2.

关 键 词：葛根素 Ⅰ型血管紧张素Ⅱ受体 血管紧张素转换酶2 高血压 

分 类 号：R285.5[医药卫生—中药学]