青海血蜱成蜱cDNA表达文库的免疫学筛选及阳性克隆分析  

Immunoscreening of cDNA expression library of Haemaphysalis qinghaiensis adult tick and analysis of positive clones

作　　者：李金杰[1] 李有全[1] 刘军龙[1] 赵海平[1] 高金亮[1] 殷宏[1] 罗建勋[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2008年第9期743-746,共4页Chinese Veterinary Science

基　　金：国家"十一五"高技术研究发展计划(863)项目(2006AA10A207);国家自然科学基金项目(30270992)

摘　　要：采用兔抗青海血蜱幼蜱阳性血清,对青海血蜱成蜱cDNA表达文库进行免疫学筛选,并对获得的阳性克隆进行序列测定及同源性分析。结果显示,共获得9个新cDNA序列,其中3个新基因序列已被GenBank接受,并获得序列接受号。表明,从青海血蜱成蜱cDNA表达文库筛选到一批新基因,为青海血蜱保护性抗原的筛选奠定了基础。cDNA expression library of Haemapkysalis qinghaiensis adult tick was immunoscreened with positive serum of rabbit against H. qingkaiensis larval tick. The obtained positive clones were sequenced and analyzed. Nine novel genes were found,and the accession numbers for three of them were obtained from GenBankTM. The result revealed that some novel genes were immunoscreened successfully,pro viding basis for the screening of protective antigens of H. qinghaiensis .

关 键 词：青海血蜱 CDNA表达文库 免疫学筛选 

分 类 号：S852.746[农业科学—基础兽医学]