三株O型口蹄疫病毒单克隆抗体的抗原识别位点分析  

Analysis of the antigenic epitope recognized by three monoclonal antibodies against foot-and-mouth disease virus type O

作　　者：宋帅[1] 林彤[1] 邵军军[1] 丛国正[1] 独军政[1] 常惠芸[1] 胡永浩[2] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,甘肃兰州730046 [2]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中国兽医科学》2008年第9期747-752,共6页Chinese Veterinary Science

基　　金：国家"十一五"科技支撑计划项目(2006BAD06A14)

摘　　要：用抗O型口蹄疫病毒(FMDV)单克隆抗体杂交瘤细胞株(4A9、4C3、9F12)制备腹水并进行纯化,经ELISA检测,单抗4A9、4C3和9F12的腹水效价分别为1∶25600、1∶102400和1∶51200,SDS-PAGE分析显示,3株纯化单抗均以IgG重链和轻链为主,其分子质量分别约为45ku和25ku。单抗4A9、4C3和9F12的饱和度分别为1∶40、1∶40和1∶1000,叠加试验所得增值指数分别为0.27%、13.05%、13.34%,均小于50%。Western-blotting结果显示,3株单抗均可与O型FMDV结构蛋白VP1发生特异性反应。表明,此3株单抗均识别O型FMDV VP1蛋白上的同一个抗原位点,存在干扰现象。The murine ascites of 3 monoclonal antibodies （4A9,4C3 and 9F12） against foot-and-mouth disease virus（FMDV） type O were produced and purified. The titers in the ascites（4Ag,4C3 and 9F12） of the mice,detected by ELISA,were 1 ： 25 600,1 ： 102 400 and 1 ： 51 200,respectively. SDS-PAGE analyses showed that IgG heavy chains and light chains of the 3 monoclonal antibodies were approximately 45 ku and 25 ku in molecular mass,respectively. The saturation degrees of the 3 monoclonal antibodies（4A9,4C3 and 9F12） were 1 ： 40,1 ： 40 and 1 ： 1 000,respectively. Their increment index,detected by competitive assay, were 0.27%, 13.05%and 13.34%,respectively, which were less than 50%. The 3 monoclonal antibodies were able to specifically react with the VP1 protein of FMDV in Western-blotting. These results indicated that the 3 monoclonal antibodies were able to recognize identical epitope of the VP1 protein of FMDV.

关 键 词：O型口蹄疫病毒 单克隆抗体 抗原位点 

分 类 号：S852.659.6[农业科学—基础兽医学]