猪瘟病毒野毒株E2蛋白特异性单抗免疫抑制制备法的建立  

Preparation of a monoclonal antibody against E2 protein of CSFV

作　　者：夏照和[1] 彭伍平[1] 成丹[1] 侯强[1] 王万利[1] 孙元[1] 仇华吉[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室猪传染病研究室,黑龙江哈尔滨150001

出　　处：《中国兽医科学》2008年第9期757-761,共5页Chinese Veterinary Science

基　　金：国家重点基础研究发展计划(973)项目(2005CB523202)

摘　　要：采用环磷酰胺免疫抑制法制备特异性识别猪瘟病毒(CSFV)野毒株的单克隆抗体,用纯化的杆状病毒表达的重组HCLV-E2蛋白作为耐受原,Shimen-E2蛋白作为免疫原,免疫BALB/c小鼠,取其脾淋巴细胞与SP2/0骨髓瘤细胞融合,获得了1株能稳定分泌抗E2蛋白单克隆抗体的杂交瘤细胞株,结果显示,该单克隆抗体能够与CSFV石门株发生反应,同时能与1.1、2.1、2.2和2.3基因亚群CSFV野毒发生特异性反应,并具有中和活性,而与CSFV兔化弱毒疫苗株不发生反应。表明,该单克隆抗体可以用于鉴别CSFV野毒株和兔化弱毒疫苗株。To preparate a monoclonal antibody（McAb） specific to the E2 protein of classical swine fever virus （CSFV） wild-type, BALB/c mice were immunized with the purified recombinant baculovirusexpressing E2 protein from C-strain（HCI.V E2） as tolerogen and with E2 protein from Shimen strain （Shimen-E2） as immunogen. The immunized murine splenic lymphocytes were fused with SP2/0 hybridoma cells. One myeloma cell line stably secreting McAb against E2 protein was obtained. The McAb could recognize CSFV Shimen strain,but not to C-strain,and could react with several subgroups,including 1.1, 2.1,2.2 and 2.3 of CSFV wild-type by the neutralizing immunofluorescence test. The results showed that the McAb could be used to differentiate CSFV wild-type strains from C-strain.

关 键 词：猪瘟病毒 E2蛋白 重组杆状病毒 单克隆抗体 免疫抑制 

分 类 号：S852.659.6[农业科学—基础兽医学] Q813.2[农业科学—兽医学]