青海省三江源地区3种生态型鼠疫耶尔森菌的基因多态性比较  

Genetics comparison on the Yersinia pestis strains of 3 kinds of ecotypes in the Sanjiangyuan area, Qinghai Province

作　　者：崔百忠[1] 

机构地区：[1]青海省地方病预防控制所鼠疫预防控制科,西宁811602

出　　处：《中国地方病学杂志》2008年第5期508-510,共3页Chinese Jouranl of Endemiology

基　　金：青海省重点科技攻关课题（2006-N-153）.志谢 本研究承蒙军事医学科学院微生物流行病研究所全军微生物检验研究中心帮助.特此致谢

摘　　要：目的对青海省三江源地区及其毗邻的四川省石渠县分离的34株3种生态型（青海田鼠型、祁连山型、青藏高原型）鼠疫耶尔森菌进行基因多态性分析，比较不同生态型鼠疫菌之间的关系。方法用随机扩增多态性DNA（RAPD）技术，对34株不同生态型鼠疫菌进行扩增，然后进行琼脂糖凝胶电泳检测。结果扩增产物在凝胶电泳上显示的条带，除3株菌不同外，其余31株菌无差异。结论三江源地区青海田鼠型、祁连山型、青藏高原型鼠疫菌在遗传学上属于同源。Objective To compare the genetic polymorphism of 34 Yersinia pestis strains of 3 kinds of ecotypes（Microtus fuscas, Qilian Mountain and Qinghai-Tibet Plateau ecotype） that isolated from the Sanjiangyuan area in Qinghai Province and Shiqu County in Sichuan Province, in order to know the ecotype relationship among the strains. Methods Thirty-four strains were amplified using the application of random amplified polymorphism DNA （RAPD） and were detected with agarose gel electrophoresis. Results Amplified products by agarose gel electrophoresis method showed same stripes in 31 strains, only 3 strains had slight differences. Conclusion The Microtus fuscus, Qilian Mountain and Qinghai-Tibet Plateau ecotype strains isolated from Sanjiangyuan area are genetically homologous.

关 键 词：随机扩增多态DNA技术 耶尔森菌 鼠疫 生态型 

分 类 号：R686[医药卫生—骨科学]