荔枝LcVSP1基因5′调控序列的克隆及功能初步鉴定  

Cloning and Function Analysis of 5′ Regulatory Region of LcVSP1 Gene from Litchi chinensis

作　　者：钟廷琼[1] 李辉亮[1] 田维敏[2] 彭世清[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]中国热带农业科学院橡胶研究所热带作物栽培生理农业部重点开放实验室,海南儋州571737

出　　处：《西北植物学报》2008年第8期1507-1512,共6页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：利用Genome Walker法获得了荔枝营养贮藏蛋白质Lc VSP1基因的5′调控序列,构建了含有该序列的植物表达载体并转化烟草,通过PCR和GUS染色对转化植株进行了鉴定.序列分析表明,Lc VSP1基因的5′调控序列中除含有真核生物典型的核心启动子区域和保守的TATA box序列外,还发现了一些与真核生物启动子中相似的顺式调控元件.对获得的转基因植株的GUS染色发现,在转基因植株的茎、叶柄和主叶脉呈现蓝色,表明Lc VSP1的5′调控序列可以启动GUS基因的表达,具有启动子的活性,并具有组织特异性.The 5′ regulatory region of LcVSP1 gene from Litchi chinensis was cloned using Genome Walker strategy. TATA box and other core configurations were found in this promoter region. Several sequences similar to eukaryotic cis regulatory element were found in the 5′ UTR proximal 5′ flanking sequence of LcVSP1. A plant expression vector with 5′ regulatory region of LcVSP1 gene from Litchi chinensis was constructed and transgenic tobacco plants were obtained by Agrobacterium-mediated transformation. GUS activity analysis revealed that the fragment of 5′ regulatory region of LcVSP1 gene drives specific expression of the GUS gene in stem,leaf stalk and leaf vein of transgenic tobacco plants.

关 键 词：荔枝 营养贮藏蛋白质 5′调控序列 顺式调控元件 

分 类 号：Q789[生物学—分子生物学]