莲品种资源的SRAP遗传多样性分析  

Genetic Diversity Analysis of Nelumbo nucifera Based on SRAP Markers

作　　者：瞿桢[1] 魏英辉 李大威[3] 肖丽舟[3] 徐金星 谢克强 周明全[1] 胡中立[1] 

机构地区：[1]武汉大学莲藕研究中心,武汉430072 [2]福建省建宁县莲子科学研究所,福建建宁354500 [3]长江大学农学院,湖北荆州434023 [4]江西省广昌县白莲科研所,江西广昌344900

出　　处：《氨基酸和生物资源》2008年第3期21-25,共5页Amino Acids & Biotic Resources

基　　金：国家自然科学基金项目(30771375);国家公益性行业科研专项(200803040);国家科技支撑计划项目(2007BAD37B06);江西省发改委以工代赈专项;江西省广昌县财政农业专项基金

摘　　要：利用分子标记SRAP技术对国内广泛栽培以及新近育成的39个莲品种进行了DNA多态性分析。选取5对引物扩增基因组DNA,共获得101条带,其中88条为多态性条带,每对引物平均提供20个标记信息。由UPMGA方法得到的聚类分析结果表明了39个品种间的遗传关系,结果表明:花莲、籽莲和藕莲三大类群有明显的界限,花莲和籽莲的遗传距离较近,藕莲与它们的遗传距离较远。分子方差分析结果表明:莲品种间和品种内均存在遗传变异,藕莲品种内的遗传变异略低于品种间的遗传变异,而诱变籽莲、诱变花莲和常规籽莲品种内遗传变异均大于品种间的遗传变异,尤其是诱变籽莲、诱变花莲品种内遗传变异占总变异的分量分别超过了70%和60%,这种品种内的遗传变异,一方面对于莲的高产稳产具有重要的意义,另一方面也说明了从这些国内广泛栽培的品种以及新近育成的莲品种中可以直接进一步选育出更优良的新品种。A newly developed molecular marker - SRAP （sequence-related amplified polymorphism） was used to detect DNA polymorphisms among the 39 lotus cuhivars. Five pair primers were used to amplify the 72 genomic DNA. A total of 101 bands were generated, 88 of them were polymorphic bands, with an average of 20 marker bands per pair of primers. The dendrogram generated by UPMGA showed the genetic relationship among 39 lotus cuhivars. There were obvious definition among three types of flower, seed and rhizome lotus. Flower lotus is close to seed lotus in genetic relationships, while rhizome lotus has the farthest relationships to the others. Genetic variation within cultivars was more than that among cultivars in induced flower lotus, general flower lotus and induced seed lotus, and it was over 70% and 60% in induced seed lotus and induced flower lotus, respectively.However, genetic variation within cultivars was less than that among cultivars in rhizome lotus. Genetic variation within cultivars is important to both high and steady yield and selecting more choiceness new eultivars from abroad planting cuhivars and freshly cultivated cuhivars.

关 键 词：莲 SRAP 亲缘关系 

分 类 号：Q3[生物学—遗传学]