栽培高粱、甜高粱和拟高粱比较基因组原位杂交分析  

Study on Comparative Genomic in situ Hybridization of S.bicolor,S.dochna and S.propinquum

作　　者：谢莉[1] 曾艳华[1] 韩永华[1] 

机构地区：[1]广西师范大学生命科学学院

出　　处：《氨基酸和生物资源》2008年第3期70-73,共4页Amino Acids & Biotic Resources

基　　金：广西自然科学基金(桂科基0448045)资助

摘　　要：用一种植物的总基因组DNA与近缘或远缘物种的染色体杂交,可以研究植物近缘或远缘物种基因组进化关系。以拟高粱总基因组DNA为探针,对栽培高粱、甜高粱基因组进行杂交,结果表明栽培高粱、甜高粱和拟高粱基因组中重复序列存在很大的同源性,基因组进化关系表现出保守性。栽培高粱与拟高粱基因组间重复序列的同源性要比甜高粱与拟高粱间重复序列的同源性高。The comparative analysis of genomic evolution between closely or distantly related species has been analyzed by a genomic DNA cross-hybridized with other species. S. propinquum genomic DNA was used as a probe cross-hybridized with S. bicolor and S. dochna. The results revealed highly homologous in repeat sequence and conserved genome evolution. S. propinquum genome cross-hybridized with S. bicolor genome showed higher homologous in repeat sequence than S. propinquum with S. dochna.

关 键 词：栽培高粱 甜高粱 拟高粱 比较基因组原位杂交 

分 类 号：Q78[生物学—分子生物学]