HPLC分析胰岛素开链反应的教学实验设计  

A Teaching Experiment on HPLC Analysis of Insulin Unfolding

作　　者：白晨[1] 吴刚[1] 张翼[1] 黄铮宇[2] 陆红[1] 

机构地区：[1]复旦大学生物科学教学实验中心生物化学实验室,上海200433 [2]复旦大学信息科学与工程学院,上海200433

出　　处：《实验室研究与探索》2008年第9期17-19,共3页Research and Exploration In Laboratory

基　　金：国家基础科学人才培养基金(J0630643)资助

摘　　要：设计DTT还原胰岛素,HPLC再现A、B链开链反应过程的生物化学实验。筛选出最适还原反应条件,最佳HPLC分离条件。通过质谱(MALDI-TOF-MS)分析确定了产物的结构。实验操作简便,结果稳定,再现性好,通过本实验学生能更好地理解和掌握HPLC的分析原理、操作方法及其在生命科学研究中的重要意义。This article designed an experiment where insulin was first reduced and unfolded by DTT, and then HPLC was used to represent the A and B chains. It developed the most probable conditions for the reduction reaction, found the best HPLC isolation method, and made a confirmation of each sample using MALDI-TOF-MS. The experiment is easy to perform, the result is invariable, and in addition, it has a desired reproducibility. It is helpful for college students to have a better understanding of the HPLC principles, to master the ways to operate it, and the importance of its role in life science research.

关 键 词：高校液相色谱 胰岛素 二硫苏糖醇 

分 类 号：Q5[生物学—生物化学]