广西产美味猕猴桃根抗肿瘤活性部位的研究  

Studies on the Antitumor Active Fraction of Roots of Actinidia deliciosa C.F.Liang et A.R.Ferguson in Guangxi

作　　者：梁洁[1] 甄汉深[1] 张薇薇[1] 李吾来[1] 王新盛[1] 黄团心[1] 

机构地区：[1]广西中医学院.药学院,广西南宁530001

出　　处：《时珍国医国药》2008年第9期2059-2061,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金地区联合资助项目(No.30160095);国家人事部留学回国人员资助项目(人发〔2002〕99号);广西新世纪十百千人才工程资助项目(No.2001209);广西高校人才小高地建设创新团队资助计划

摘　　要：目的研究广西产美味猕猴桃根抗肿瘤活性部位。方法利用系统溶剂分离法,对美味猕猴桃根进行部位分离,对其70%乙醇总提取物、石油醚提取物、醋酸乙酯提取物、正丁醇提取物和95%乙醇提取物采用MTT法测定它们各自对宫颈癌细胞株Hela,人胃癌细胞株SGC7901,黑色素瘤细胞株B16,人肝癌细胞株Hele7404,小鼠肉瘤细胞株S180和小鼠肝癌细胞株H226种瘤株的生长抑制情况。结果美味猕猴桃根的70%乙醇总提取物、醋酸乙酯提取物和正丁醇提取物对6种瘤株有明显的抑制作用。结论美味猕猴桃根的70%乙醇总提取物、醋酸乙酯提取物和正丁醇提取物具有一定的体外抗肿瘤作用。Objective To study the antitumor active fraction of roots of Actinidia deliciosa C. F. Liang et A. R. Ferguson in Guangxi. Methods The chemical constituents of roots of Actinidia deliciosa were extracted and isolated by systematic solvents. The 70% alcoholic extracts were divided into four portions including petroleum ether extract, ethyl acetate extract, n - Butyl alcohol extract and 95% alcoholic extract. Antitumor experiment of different extracts in vitro was done by MTT assay on human uterine carcinoma Hela, human gastric adenocarcinoma SGC7901, B16 melanoma cell, human hepatoma Hele7404, mouse entity tumor S180 and hepatoma H22- Results The growth of all tumor cells were inhibited by 70% alcoholic extract, ethyl acetate extract and n - Butyl alcohol extract from roots of Actinidia deliciosa. Conclusion 70% alcoholic extract, ethyl acetate extract and n - Butyl alcohol extracts have some antitumor effects in vitro.

关 键 词：广西产美味猕猴桃根 抗肿瘤作用 活性部位 

分 类 号：R285.5[医药卫生—中药学]