粗根荨麻水提取部分对AA大鼠腹腔巨噬细胞PGE_2水平及体外LPS诱导腹腔巨噬细胞COX-2 mRNA表达的影响  

Effects of Aqueous Fraction of Urtica macrorrhiza Hand-Mazz on Production of PGE_2 Release from Peritoneal Macrophages in Vivo and the Expression of COX-2 mRNA in Vitro

作　　者：李晓红[1] 李顺英[1] 赵永娜[1] 李兵兰[1] 邵晓霞[1] 

机构地区：[1]昆明医学院.药学院,云南昆明650031

出　　处：《时珍国医国药》2008年第9期2132-2134,共3页Lishizhen Medicine and Materia Medica Research

基　　金：云南省教育厅科学技术基金重点项目(No.04Z294C)

摘　　要：目的观察滇产粗根荨麻水提取部分Urtica macrorrhiza Hand-Mazz,Ur对佐剂性关节炎(adjuvant arthritis,AA)大鼠腹腔巨噬细胞(peritoneal macrophages,PMφ)分泌前列腺素E2(prostaglandin E2,PGE2)水平及对体外LPS诱导PMφCOX-2 mRNA表达的影响,探讨其治疗类风湿性关节炎可能的作用机制。方法建立AA大鼠模型,Ur水提取部分连续灌胃给药14 d或21 d后分次获取PMφ,LPS诱导PGE2的产生,用酶联免疫吸附法检测培养上清液中PGE2水平。体外培养PMφ,RT-PCR方法检测LPS诱导COX-2 mRNA表达。结果Ur水提取部分(400,200 mg/kg)对LPS诱导的AA PMφ分泌PGE2水平有明显抑制作用。Ur(200,100μg/ml)能显著抑制LPS诱导的COX-2 mRNA的表达。结论滇产粗根荨麻水提取部分对佐剂性关节炎的治疗作用可能与其抑制PMφ分泌PGE2及COX-2 mRNA表达有关。Objective To investigate the effects of aqueous fraction of Urtica macrorrhiza Hand- Mazz（Ur） on modulating prostaglandin E2 （ PGE2 ） production in vivo and the expression of COX - 2 mRNA in vitro and elucidate the possible mechanisms of anti -inflammatory and anti -rheumatoid effects of Ur. Methods Adjuvant arthrifis（AA） rat was used as the model. The PMφ samples were taken at different time after medication. PGE2 level was measured by ELISA method. PMφ induced by lipopolysaccharide（LPS） was cultured in vitro, and the expression of COX -2 mRNA was detected by RT - PCR. Results Ur（400 mg/kg and 200 mg/kg） could inhibit LPS induced PGE2 release from PMφ in AA rats. The expression of COX -2 mRNA induced by LPS was significantly increased. Ur（200 μg/ml and 100 μg/ml） could decrease the expression of COX -2 mRNA . Conclusion The anti - inflammatory mechanisms of Ur in AA rats might be related to its inhibitory effects on the level of PGE2 from PMφ in vivo and the expression of COX - 2 mRNA in vitro

关 键 词：粗根荨麻 佐剂性关节炎 前列腺素E2 COX-2MRNA 

分 类 号：R962[医药卫生—药理学]