高效液相色谱法测定参杞益脑胶囊中β-细辛醚的含量  

Determination of β-asarone in Shenqiyinao Capsules by HPLC

作　　者：严全鸿[1] 饶春意[1] 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《时珍国医国药》2008年第9期2222-2223,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立了参杞益脑胶囊中β-细辛醚的高效液相色谱含量测定方法。方法色谱柱为Thermo BDS Hypersil C18（250mm×4．6mm，5μm）；流动相为甲醇-0．1％冰醋酸溶液（60：40）；柱温35℃；流速1．0ml·min；检测波长为257nm。结果β-细辛醚浓度在4．885～146．55μg/ml之间峰面积与浓度具有良好的线性关系。其高、中、低3种浓度的平均回收率分别为103．2％，102．1％和100．2％；RSD分别为0．4％，0．3％和0．7％。结论方法简便灵敏，结果准确可靠，专属性强，可用于参杞益脑胶囊中石菖蒲的质量控制。ObjectiveTo establish a high performance liquid chromatographic method for the determination of β- asarone in Shenqiyinao Capsules. Methods The column was Thermo BDS Hypersil C18 （250 mm ×4.6 mm ,5 μm）. The mobile phase was methanol - 0.1% glacial acetic acid solution （60： 40） and the temperature of column was 35 ℃. The flow rate was 1.0 ml · min^-1 and the detection wavelength was 257 nm with external method. Results The linear range of β - asarone was 4. 885 - 146.55 μg · ml^-1. The average recoveries of β- asarone were 103.2% , 102.1% and 100.2% with corresponding RSD of 0.4% , 0.3% and 0.7% , respeetivery. Conclusion The method is simple, sensitive, accurate and can be used for the determination of Rhizoma Acori Tatarinowii （RAT） in Shenqiyinao Capsules.

关 键 词：参杞益脑胶囊 Β-细辛醚 石菖蒲 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]