苦瓜总皂甙含量测定及超声提取最佳条件的研究  

Spectrophotometric Determination of Total Saponins from Momordica charantia and Condition Optimization of Ultrasonic Extraction

作　　者：邱红[1] 魏佑营[1] 王军伟[1] 魏秉培[1] 曹克友[1] 吴静[1] 

机构地区：[1]山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安271018

出　　处：《山东农业科学》2008年第6期86-89,共4页Shandong Agricultural Sciences

基　　金：泰安市大学生科技创新引导计划项目

摘　　要：建立了苦瓜总皂甙的超声提取和分光光度测定方法,并优化了超声提取的提取温度、乙醇浓度、料液比和提取时间等因素。结果显示,超声波提取法最佳工艺组合为:70%乙醇,液料比为30∶1,振荡频率为60%,70℃超声提取30 m in/次,重复提取2次;且分光光度测定法操作简便、快速、准确,可作为苦瓜制品提取及质量评价方法。The methods for ultrasonic extraction and spectrophotometric determination of total saponins from Momordica charantia were established;the extracting temperature, alcohol content, the ratio of material to solvent, extracting time and so on were also optimized. The results showed that the optimum conditions for ultrasonic extraction were 30：1 of 70% alcohol solution to Mormordica charantia weight, extraction for 2 times repeatedly and 30 minutes every once under 70℃ with 60% oscillation frequency. The method is simple, rapid and accurate and suitable for the quality evaluation of Momordica charantia material and products.

关 键 词：苦瓜 总皂甙 超声提取 分光光度法 

分 类 号：O657.3[理学—分析化学]