家蚕滞育激素受体基因(BmDHR)的分子克隆及定量分析  

Cloning and Quantitative Analysis of Diapause Hormone Receptor Gene in the Silkworm,Bombyx mori

作　　者：顾燕燕[1] 华荣胜[1] 周耐明[2] 时连根[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江大学生命科学学院,杭州310058

出　　处：《蚕业科学》2008年第3期417-423,共7页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(编号30571406)

摘　　要：家蚕滞育激素受体(BmDHR)能特异性地识别滞育激素(DH),参与滞育激素信号的转导,在家蚕滞育发动过程中发挥重要作用。采用RT-PCR方法克隆出BmDHRcDNA,其开放阅读框长1 311 bp,编码436个氨基酸,属于一种G蛋白偶联受体,含有7个跨膜结构域。同源性及系统进化分析表明,BmDHR蛋白序列与玉米夜蛾性信息素合成激活肽受体(HzPBANR)和家蚕性信息素合成激活肽受体(BmPBANR)蛋白序列具有较高的同源性,分别为46.0%和40.9%。定时半定量分析显示,BmDHR基因表达水平在不同条件催青的二化性5龄幼虫、蛹体中存在差异。Diapause hormone receptor of Bombyx mori （BmDHR） could recognize diapause hormone （DH） specifically and participate in the process of diapause signal transduction, so it plays an important role in the initiation of diapause. In this paper, BmDHR cDNA was cloned by RT-PCR, and the open reading frame of cDNA was 1 311 bp in length, encoding 436 amino acids. BmDHR belonged to the G protein-coupled receptor with 7 transmembrane domains. The homologous and systematic evolution analysis results showed that the deduced amino acids of BmDHR had 46. 0% and 40.9% identical to that of pheromone biosynthesis activating neuropeptide receptor of Helicoverpa zea （HzPBANR） and pheromone biosynthesis activating neuropeptide receptor of Bombyx mori （ BmPBANR）, respectively. Semi-quantitative analysis results showed that expression level of BmDHR was different in 5th instar larvae and pupa of bivoltine race incubated under different environment conditions.

关 键 词：家蚕 滞育激素受体 基因克隆 半定量分析 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]