双层壳聚糖/羟基磷灰石复合支架的制备及性能研究  

Preparation and Physicochemical Properties of Chitosan/Hydroxyapatite Bilayered Scaffold

作　　者：张华亮[1] 王文良[2] 关静[3] 刘英杰[2] 初殿伟[2] 张西正[3] 

机构地区：[1]武警九江市支队卫生队,江西九江332000 [2]武警医学院附属医院,天津300162 [3]军事医学科学院卫生装备研究所,天津300161

出　　处：《医疗卫生装备》2008年第9期4-6,11,共4页Chinese Medical Equipment Journal

基　　金：国家自然科学基金项目(30740089);武警医学院科研基金项目(WBS2007-13)

摘　　要：目的:探索制备一种壳聚糖(CS)/羟基磷灰石(HA)双层复合支架的方法,对支架的理化性质进行检测。方法:在3%(m/V)壳聚糖溶液中加入一定量的聚乙二醇,离心去除气泡。将HA支架放入模具中,取配好的壳聚糖溶液,倒入模具中,放入冰箱冷冻,在冷冻抽干机中冻干,制成HA/壳聚糖(CS)支架。采用外观、红外光谱、电镜、乙醇替代、生物力学测定等方法检测支架的材料、内部结构(孔径、孔相通性)、孔隙率、力学性能,考察将其作为骨软骨组织工程支架材料应用的可行性。结果:双层复合支架外观上模拟骨软骨组织学结构,材料为壳聚糖、羟基磷灰石,平均孔径分别为CS层300μm,HA层350μm,孔相通性好,CS孔隙率为(76.00±5.01)%,HA孔隙率为(72.00±4.23)%。CS支架弹性模量为(2.8±0.5)MPa,HA支架的弹性模量为(150±11)MPa。结论:壳聚糖(CS)/羟基磷灰石(HA)双层复合支架符合骨软骨组织工程支架要求,具有较好的应用前景。Objective To prepare and test the physicochemical properties of chitosan （CS） / hydroxyapatite （HA） bilayered scaffold.Methods The bilayered HA/CS scaffolds were fabricated by placing the HA scaffolds into cylindrical moulds and transferring the 3%（m/V） CS solution. Moulds were frozen and lyophilized to remove the frozen solvent. Physicochemical characteristics such as material, inner structure, porosity and mechanical property were examined by FTIR, SEM, method of liquid displacement and mechanical testing respectively. Results Bilayered scaffold simulated osteochondral histologic structure in the appearance. SEM micrograph showed CS pore at the typical transversal cross-section of CS layer with open pores and pore size with average 300 μm and HA pore at the typical transversal cross-section of HA layer with open pores and pore size with average 350 μm. Physicochemical characterization of CS/HA bilayered scaffold： HA possessed a porosity of （76.00 ±5.01 ）%; CS layer possessed a porosity of （72.00±4.23）%. The modulus（E） for the HA and CS in compression were found to be （150 ±11） MPa and （2.8 ± 0.5） MPa respectively. Conclusion CS/HA bilayered scaffold meets the requirements of osteochondral tissue-engineering scaffold and has bright application future.

关 键 词：骨软骨组织工程 壳聚糖 羟基磷灰石 

分 类 号：R318.08[医药卫生—生物医学工程]