紫杉醇脂质体的制备及质量考察  

Preparation and Quality Study of Paclitaxel Liposome

作　　者：康艳敏[1] 吴燕[2] 杨长青[1] 吴世福[3] 梅兴国[2] 

机构地区：[1]延边大学药学院,延吉市133000 [2]军事医学科学院毒物药物研究所,北京市100850 [3]沈阳中药制药有限公司,沈阳市110044

出　　处：《中国药房》2008年第28期2208-2210,共3页China Pharmacy

摘　　要：目的:制备紫杉醇脂质体并考察其质量。方法:以薄膜分散法制备脂质体;采用高效液相色谱法测定其中主药的含量并计算包封率及体外释放度。结果:所制脂质体粒径为70～150nm;紫杉醇检测浓度的线性范围为0.3～75μg·mL-1(r=0.9998),平均回收率为100.3%(RSD=1.16%,n=3);包封率约为96.46%;体外释药符合Higuchi方程,具有缓释性。结论:薄膜分散法适于制备紫杉醇脂质体;离心法能够准确快速测定脂质体包封率;该制剂体外缓慢释药。OBJECTIVE： To prepare paclitaxel liposome and study its quality. METHODS： Paclitaxel liposome was prepared by film dispersion method, and the content of paclitaxel was determined by HPLC. The encapsulation efficiency （EE） and the in vitro release rate of liposome were computed as well. RESULTS： The particle size of the prepared liposome ranged from 70 mn to 150 nm and its EE% was 96.46%. The linear range of paclitaxel was 0.3-75 ug · mL ^-1（r=0.999 8） with an average recovery rate of 100.3% （RSD = 1.16%, n = 3） . The release rate of paclitaxel liposome in vitro fitted to Higuchi model and was characterized by slow release. CONCLUSIONI The film dispersion method is suitable for the preparation of paelitaxel liposome. This drug has a slow release property in vitro.

关 键 词：紫杉醇脂质体 薄膜分散法 包封率 

分 类 号：R927.2[医药卫生—药学] R979.1