反相高效液相色谱法测定甘草酸18H-差向异构体含量  

Determination of Two 18-Epimers of Glycyrrhizin by RP-HPLC

作　　者：王佩[1] 刘晓昱[1] 吴锡铭[2] 

机构地区：[1]浙江省杭州市中医院,浙江杭州310007 [2]浙江省杭州市第六人民医院,浙江杭州310014

出　　处：《中国药业》2008年第19期17-18,共2页China Pharmaceuticals

摘　　要：目的建立同时测定18α-甘草酸和18β-甘草酸的反相高效液相色谱法。方法色谱柱为C18柱(250mm×4.6mm,5μm),流动相为0.1mol/L磷酸盐缓冲液(pH=7.0)-乙腈(80:20),流速1.0mL/min,柱温30℃,检测波长250nm。结果18α-甘草酸和18β-甘草酸达到基线分离,两者的进样量在1.0～2.0μg范围内与峰面积线性关系良好,r=0.9998(n=6),平均回收率分别为99.58%和99.94%,RSD分别为0.54%和0.28%(n=9)。结论所用方法简便、快速,可同时测定18α-甘草酸和18β-甘草酸的含量。Objective To establish a RP-HPLC method for the determination of 18α-glycyrrhizin （18a-GL） and 18β-glyeyrrhizin （18β-GL）. Methods Phenomenex C18 column （250 mm ×4. 6 mm, 5 μm） was used as the stationary phase with acetonitrile-0. 1 mol/L phosphate buffering solution （20：80） as the mobile phase and the detection wavelength was at 250 nm. Results The calibration curves were linear in the range of 1.0-2.0 μg for 18α-GL and 18β-GL, r=0.999 8（n=6）.The average recovery rate was 99.58% with RSD O. 54% and 99.94% with RSD 0. 28% for 18α-GL and 18β-GL respectively. Conclusion This method, is easy and faster for the quantitative determination of 18α-GL and 18β-GL.

关 键 词：18Α-甘草酸 18Β-甘草酸 差向异构体 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R975.5