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机构地区:[1]重庆市药品检验所,重庆401121
出 处:《中国药业》2008年第19期23-24,共2页China Pharmaceuticals
摘 要:目的建立当归拈痛丸中黄芩苷的含量测定方法。方法采用反相高效液相色谱法,色谱柱为C18柱(200mm×4.6mm,5μm),甲醇-0.2%磷酸溶液(45:55)为流动相,流速为1.0mL/min,检测波长为278nm,柱温35℃。结果黄芩苷进样量在0.06084~1.8252μg范围内与峰面积线性关系良好,r=1.00,平均加样回收率为97.0%,RSD为1.1%(n=9)。结论反相高效液相色谱法灵敏、简便、准确、重现性好,适用于当归拈痛丸的质量控制。Objective To establish a RP- HPLC method for the determination of baicalin in Danggui Niantong Pills. Methods RP- HPLC method was adopted and the C18 column (200 mm×4.6 mm,5 μm) was used at 35 ℃. The mobile phase was methanol-0. 2% phosphoric acid (45:55) with the flow rate of 1.0 mL/min. The detection wavelength was at 278 nm and the column temperature was 35℃. Results The calibration curve of baicalin was linear in the range of 0. 060 84 - 1. 825 2 μg. The average recovery rate was 97.0% , RSD = 1.1% (n =9). Conclusion The method is highly sensitive, quick and accurate with good repeatability. It can be used for the quality control of Danggui Niantong Pills.
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