Study on the Integration of T-DNA into Lilium longiflorum  

T-DNA插入麝香百合的研究(英文)

作　　者：李刚[1] 刘菊华[1] 谭光兰[1] 徐碧玉[1] 金志强[1] 

机构地区：[1]中国热带农业科学院生物技术研究所

出　　处：《Agricultural Science & Technology》2008年第2期33-35,41,共4页农业科学与技术（英文版）

基　　金：the Fund of Basis Scientific Research Operation of Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;the Grant of Scientific Fund of Chinese Academy of Tropical Agricultural Sciences (NoRky0529)~~

摘　　要：[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [ Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 -0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily proving that T-DNA gene had integrated into the genome of lily. [ Conclusion] The study may lay a foundation for breeding excellent lily varieties through TDNA integration technique.采用2步外植体法结合比较成熟的农杆菌介导T—DNA插入技术转化麝香百合，为快速选育百合新品种奠定基础。

关 键 词：Lilium longiflorum Agrobacterium-mediated transformation T-DNA Insertion mutation 

分 类 号：S682.29[农业科学—观赏园艺]