结核分枝杆菌链霉素耐药基因rpsL的检测  

Detection of the streptomycin-resistant rpsL gene from Mycobacterium tuberculosis

作　　者：邵明明[1] 薛欣[1,2] 楚雍烈[1] 寻萌[1] 宋娟[1] 曹春霞[1] 邱奕 

机构地区：[1]西安交通大学医学院病原生物学教研室,陕西西安710061 [2]西安市结核病医院,陕西西安710061

出　　处：《中国病原生物学杂志》2008年第9期651-653,共3页Journal of Pathogen Biology

基　　金：西安市科技创新支撑计划项目(No．YF07158)。

摘　　要：目的探讨结核分枝杆菌(TB)对链霉素(Sm)耐药的分子机制,建立快速检测结核分枝杆菌Sm耐药性的方法。方法应用聚合酶链反应-单链构象多态性(PCR-SSCP)和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测80株结核分枝杆菌临床分离株的rpsL基因。结果以H37Rv标准株为对照,21株敏感株中,1株(4.76%) SSCP和RFLP分析存在rpsL基因异常;59株耐药株中,36株(61.02%)SSCP和RFLP分析存在rpsL基因异常。结论结核分枝杆菌对Sm耐药与rpsL基因突变有关,PCR-SSCP和PCR-RFLP技术可快速、准确地检测结核分枝杆菌对Sm的耐药性。Objective To study the molecular mechanism of Mycobacterium tuberculosis resistance to streptomycin, and establish a rapid detective method of rpsL gene mutation in M. tuberculosis. Methods The rpsL genes of 80 mycobaeterium tuberculosis clinical isolates were detected by PCR-SSCP and PCR-RFLP. Results Strain H37Rv was used as the control. In 80 mycobacterium tuberculosis clinical isolates, 1 of 21 （4.76%） drug susceptible isolates and 36 of 59 （61.02 % ） streptomycin-resistant isolates showed rpsL gene mutation by SSCP and RFLP. Conclusion Drug resistance of M. tuberculosis to streptomycin is related to rpsL gene mutation, PCR-SSCP and PCR-RFLP might become a rapid detecting method of streptomycin resistance of M. tuberculosis.

关 键 词：分枝杆菌 结核 耐药 基因突变 多态性 单链构象 限制性片段长度 

分 类 号：R378.911[医药卫生—病原生物学]