盖尔森基兴奴卡氏菌的生物学特性研究  

Biological characteristics of Nocardia cyriacigeorgica

作　　者：陶元勇[1] 苏芬[2] 黄英[3] 李丽丽 赵乃昕[5] 

机构地区：[1]潍坊医学院附属医院,山东潍坊261031 [2]潍坊市人民医院 [3]中国科学院微生物研究所 [4]山东大欣微生物研究院 [5]潍坊医学院

出　　处：《中国病原生物学杂志》2008年第9期654-656,共3页Journal of Pathogen Biology

摘　　要：目的研究盖尔森基兴奴卡氏菌的生物学特征、分子生物学特性及其与感染的关系。方法在CT导引下作肺穿刺取得标本,经培养获得革兰阳性、生长较慢的丝杆状菌,进行形态、培养特征、细胞壁化学成分分析和16S rRNA基因测序。结果经形态学、培养特征、细胞壁化学成分分析和16S rRNA基因测序,鉴定为盖尔森基兴奴卡氏菌。测定的1 429 bp序列与此种菌的模式株的序列100%一致,分离菌株与模式株的生化反应仅有两项不同。结论本文全面报道盖尔森基兴奴卡氏菌的生物学特性。Objective To study the biology characteristic and molecular biology characteristic and the relations with infection of Nocardia cyriacigeorgica. Methods The gram-positive, slow-growing silk-form bacillus was obtained from the cultured specimen collected by lung puncture in CT, and the morphological, culture characteristics, cell-wall chemical composition and 16s RNA gene sequence were analyzed. Results The microorganism was identified as N. cyriacigeorgica by the morphological, culture characteristics, cell-wall chemical composition analysis and 16sRNA gene sequencing. The sequenced gene fragment （1 429 bp） of the isolated strain was identical （100%） to the normal strain of N. cyriacigeorgica. Only two biochemical differences were founded between the isolated strain and normal strain. Conclusion The biological characteristics of Nocardia cyriacigeorgica was determined.

关 键 词：韦格纳氏肉芽肿 盖尔森基兴奴卡氏菌 肺感染 16S RRNA基因序列 

分 类 号：R378.99[医药卫生—病原生物学]