降落PCR扩增单纯疱疹病毒Ⅱ型D蛋白基因的研究  

Study on detection of gD DNA of herpes simplex virus type Ⅱ by TD-PCR

作　　者：刘涛[1] 黄志成[1] 祝水芬[1] 

机构地区：[1]杭州市疾病预防控制中心微生物检验科,杭州310006

出　　处：《中国卫生检验杂志》2008年第9期1735-1736,共2页Chinese Journal of Health Laboratory Technology

基　　金：杭州市科技发展计划项目(20080333Q29)

摘　　要：目的:建立一种快速、准确检测单纯疱疹病毒Ⅱ型D蛋白基因的方法。方法:根据已发表的gD蛋白核苷酸序列设计引物,采取降落PCR对gD蛋白DNA进行扩增。结果:从2份病毒株中均扩增出与预期大小完全一致的目的片段,并且采用降落PCR能有效地降低或避免非特异性扩增。结论:TD-PCR能够用于快速,准确地检测gD蛋白,为生殖器疱疹的鉴别诊断在分子水平上提供了理想的新方法。Objective：To establish a method which can test instantly and precisely the protein D gene of herpes simplex virus type Ⅱ.Methods：After designing a primer according to the published protein D nucleotide sequence,the DNA of protein D was amplified with the touchdown PCR（TD-PCR）.Results：The objective segments were amplified from two herpes simplex virus type Ⅱ strains whose sizes were exactly the same as expected,and it could reduce or avoid the non-specific amplification effectively.Conclusion：The TD-PCR can be used for instant and precise test of protein D.It provides a new and ideal method for the genital herpes diagnosis at the level of molecule.

关 键 词：TD-PCR gD蛋白 单纯疱疹病毒Ⅱ型 

分 类 号：Q78[生物学—分子生物学]