薄层色谱扫描法快速检测乌头碱中毒  

Rapid diagnosis of aconites poisoning by TLCS

作　　者：尉志文[1] 刘洁 贠克明[1] 王玉瑾[1] 贾娟[1] 

机构地区：[1]山西医科大学法医学院,太原030001 [2]山西省临汾市公安局尧都分局,山西临汾041000

出　　处：《中国卫生检验杂志》2008年第9期1746-1747,共2页Chinese Journal of Health Laboratory Technology

基　　金：国家十一五科技支撑计划项目(2007BAK26B05)

摘　　要：目的：建立乌头碱中毒的薄层色谱快速检验方法。方法：样品加乙醚,（NH4）2SO4后调pH到10,分出有机层加盐酸,弃去乙醚层,水层加二氯甲烷和氨水提取,挥干后20μl乙醇定容,10μl点于HPGF254板上,环己烷：乙酸乙酯：二乙胺（8：1：1）饱和上行展开5-7 cm,薄层色谱扫描定性和定量检测。结果：乌头碱线性范围为1-32μg/ml,新乌头碱与次乌头碱均为4-28μg/ml,最小检出浓度0.1μg/ml。结论：本法快速、简便,定性定量准确,可用于乌头碱中毒的快速诊断和乌头碱中毒致死的法医学鉴定。Objective：To develop a rapid qualitative and quantitative analysis method for aconites poisoning by TLCs.Methods：The matrix was extracted twice by ether,（NH4）2SO4,dichlormethane and ammonia water.Evaporated in a water bath and with an air velocity of nitrogen gas,extract was then dissolved with ethanol and analyzed by TLCs.Results：The extraction curves of aconites was linear over the range of 1～32 μg/ml,the extraction curves of new-aconites and hypo-aconites were linear over the range of 4～28 μg/ml.The limit of detection was 0.1 μg/ml.Conclusion：The analysis is more rapid,simpler,more sensitive and more precise.It is a better method for diagnosis of aconites poisoning and forensic identification of aconites poisoning death.

关 键 词：乌头碱 新乌头碱 次乌头碱 薄层色谱法 

分 类 号：O658[理学—分析化学]