程序冷冻法、液氮蒸汽法冷冻人类精子的效果比较  

Comparision of effects of two methods on cryopreservation of human sperm

作　　者：李玉山[1] 王全先[1] 高学敏[1] 杨险峰[1] 胡耀龙[1] 

机构地区：[1]郑州大学第三附属医院人类精子库,郑州450052

出　　处：《中国卫生检验杂志》2008年第9期1814-1815,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:比较程序冷冻法和液氮蒸汽法冷冻保存人类精子的效果。方法:用同一批次GYC型冷冻保护剂,分别应用程序冷冻法和液氮蒸汽法对68份精液标本进行冷冻保存,并比较其冷冻复苏率。结果:应用程序冷冻法冷冻的精液标本,其复苏后前向运动精子(a+b级)的冷冻复苏率为66.7%±8.1%,应用液氮蒸汽法冷冻的精液标本,其复苏后前向运动精子(a+b级)的冷冻复苏率为63.9%±7.9%,两组相比无显著差异(P>0.05)。结论:采用程序冷冻法和液氮蒸汽法冷冻人类精子,二者冷冻复苏率无显著差异,两种方法均可以用于人类精子库冷冻保存精液标本。Objective：To compare the effect of programmable freezer and liquid nitrogen vapour method on the cryopreservation of human sperm.Methods：A total of 68 specimen samples were frozen by program freezing method and liquid nitrogen vapour method with the same cryopreservative media to compare the post-thaw sperm motility.Results：The forward motility（a＋b） rate of post-thaw human sperm by program freezing method was 66.7%±8.1%,and by liquid nitrogen vapour method 63.9%±7.9%.There was no significant difference between the two cryoprotective methods（P〉0.05）.Conclusion：Both program freezing method and liquid nitrogen vapour method can be used to cryopreserve human sperm.

关 键 词：精液保存 冷冻精子 程序冷冻法 液氮蒸汽法 

分 类 号：R322.6[医药卫生—人体解剖和组织胚胎学]