中药复方对戊四唑致癫小鼠海马神经元型一氧化氮合酶表达的影响  

Effect of traditional chinese medicinal compound on expression of nNOS in hippocamp of epileptogenic rat treated by phrenazol

作　　者：秦晓勇[1] 詹曦菁[2] 熊杰[1] 

机构地区：[1]武警医学院附属医院中医理疗科,天津300162 [2]武警医学院微生物教研室,天津300162

出　　处：《中国卫生检验杂志》2008年第9期1832-1833,1864,共3页Chinese Journal of Health Laboratory Technology

基　　金：武警总部课题(WKH2001092)

摘　　要：目的:探讨中药复方对戊四唑致痫小鼠脑诱导型神经元一氧化氮合酶(nNOS)表达的影响。方法:40只昆明种小鼠,按随机方法分为4组,即中药复方2个剂量组、模型组、正常对照组。将两组不同浓度的中药复方浓缩剂,分别灌服两组小鼠,连续6 d。前3组腹腔注射戊四唑(55 ml/kg)致痫,55 min后处死动物。采用免疫组织化学法,观察中药复方对戊四唑致痫小鼠nNOS表达的影响。结果:中药复方各组nNOS阳性细胞数目明显多于模型组(P<0.01)。中药复方两个剂量组亦存在差异(P<0.05)。结论:中药复方对癫痫小鼠海马神经元有保护作用,此结果可能是其治疗癫痫的机理之一。Objective：To study the effect of traditional Chinese medicinal compound on the expression of neuron type nitricoxide synthase（nNOS） in hippocamp of epileptogenic rat treated by pentylenetetrazol（PTZ）.Methods：Forty rats were randomly divided into four groups,including two experimental groups,one model group and one normal control group.The rats in experimental groups were fed with two concentration drugs respectively for 6 days.Then experimental and model groups were all intraperitoneal injected with PTZ to modeling epilepsy.The animals were sacrificed 55 minutes later,then immunohistochemical methods were used to show the change of nNOS in hippocamp.Results：The number of nNOS positive neurons in experimental group rats is more than that of model group（P〈0.01）,and there is also difference in the number of nNOS positive neurons between two experimental groups（P〈0.05）.Conclusion：Traditional Chinese medicinal compound has protective function on related nerve cells of epileptogenic rats.The results may be one of the mechanisms of the drug in curing epileptics.

关 键 词：中药复方 癫痫小鼠 神经元型一氧化氮合酶 戊四唑 

分 类 号：R285[医药卫生—中药学]