增菌荧光定量PCR检测痰及血液中结核杆菌的临床应用  

Clinical application of bacteria proliferation accompanied PCR for detection of Mycobacteria tuberculosis in sputum and blood

作　　者：张永乐[1] 朱明利[1] 张利诚[2] 娄国强[1] 

机构地区：[1]杭州市第六人民医院浙江省中医药大学附属杭州第六医院开放实验室,杭州310014 [2]武警浙江省总队医院呼吸科,浙江嘉兴314000

出　　处：《中国卫生检验杂志》2008年第9期1834-1835,共2页Chinese Journal of Health Laboratory Technology

基　　金：杭州市医药卫生科技计划项目(2003B041)

摘　　要：目的:评价增菌荧光定量PCR(FQ-PCR)检测痰及血液中结核杆菌的临床应用价值。方法:对61例菌阴和难治性结核患者的痰液、血液以及增菌痰液、增菌血液分别进行FQ-PCR检测,比较相互之间的差异性。结果:痰液、增菌痰液FQ-PCR检测阳性率分别为27.87%(17/61)、40.43%(19/47),二者比较2χ=3.92,P<0.05,差异有统计学意义。血液、增菌血液FQ-PCR检测阳性率分别为0(0/35)、22.5%(9/40),二者比较2χ=8.94,P<0.005,差异有统计学意义。痰液总阳性率为45.90%(28/61),较血液总阳性率22.5%(9/40)高,二者比较2χ=5.70,P<0.05,差异有统计学意义。结论:增菌FQ-PCR在菌阴性和难治性结核杆菌的检测中可作为结核病的辅助诊断之一。痰液FQ-PCR阳性率明显高于血液,但痰液、血液联合FQ-PCR检测可提高结核杆菌的检出率。Objective：To determine the clinical value bacteria for detection of MTB FQ-PCR in sputum and blood.Methods：From 61 patients of negative TB sputum,blood or sputum by bacteria proliferation,conducted by FQ-PCR,and compare the differences between each other.Results：The positive rates of FQ-PCR before and after bacteria proliferation were 27.87%（17/61）,40.43%（19/47） compared the two group χ2=3.92,P〈0.05,the difference is statistically significant.Blood and blood of bacteria proliferation FQ-PCR positive rates were 0（0/35）,22.5%（9/40）,χ2=8.94,P〈0.005,the difference statistically significant.The total positive rate of sputum is 45.90 percent（28/61）,and blood is 22.5%（9/40）,χ2=5.70,P〈0.05,the difference is statistically significant.Conclusions：Bacteria proliferation accompanied FQ-PCR for detection of Mycobacteria tuberculosis can be used as the supplementary diagnosis of negative bacteria and refractory tuberculosis Sputum FQ-PCR positive rate was significantly higher than that of blood,sputum,blood joint FQ-PCR detection met had can increase the detection rate of TB.

关 键 词：增菌 荧光定量聚合酶链反应 分枝杆菌 结核 

分 类 号：R378.911[医药卫生—病原生物学]