放射性^(125)碘对移液器容量的比对与校准  

Checking and calibration of pipette volume with radioisotopic iodine-125 labeling

作　　者：王志刚[1] 邵平扬[1] 曹浩强[1] 

机构地区：[1]浙江省嘉兴市第二医院,浙江嘉兴314000

出　　处：《中国卫生检验杂志》2008年第9期1838-1841,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立精确有效、简便快速的同位素标记法用于实验室移液器容量的比对与校准,实现移液器准确度与精确度的有效监控。方法:以125I标记溶液为示踪材料,以放射性γ计数仪为设备,以测定CPM值为观察指标,用已通过重量分析法检定的移液器为标准,其他待检移液器容量通过放射性同位素溶液吸样测定CPM与之比较进行方法学评价。结果:放射性同位素法检测批内精密度在0.88%~1.08%之间;平均回收率98.83%;CPM值与125I浓度梯度及容量之间关系的回归方程分别为:Y=23879X-388.4(r2=0.9992)和Y=572.18X-175.83(r2=0.9994)呈正相关;标准移液器与待检移液器的容量平行试验结果良好。结论:放射性同位素125I标记进行移液器容量比对与校准技术,方法灵敏、精确、简便,可适用于临床实验室。Objective：To establish the sensitivity,efficiency,convenient,and rapid radioisotopic iodine-125-labeled method for volumes checking and calibration,which monitoring the accuracy and precision for the contains of pipettes.Methods：We employs an ^125I solution and experiment instrument for evaluation the new method.The delivery volume of one pipette is checked by gravimetric analysis.Than the remaining anticipate pipettes are compared by dispensing ^125I solution.Measuring the counts per minute（CPM） and calculate the mean,SD and CV% to ensure that the accuracy and precision were within or outside the acceptable range.Results：The inter-assay imprecision were 0.88%-1.08%.The average rate of spiking recovery was 98.83%.The regression equation for a linear curve between CPM and gradient dilution concentrations of ^125I or volumes were：Y=23879X-388.4（r^2=0.9992）and Y=572.18X-175.83（r^2=0.9994）revealed positively correlated.Paralleled comparative analysis of the certified pipette and the anticipate pipettes were performed good.Conclusion：We presented a novel radioactive ^125I-labeled methodology,which combines highly sensitivity,accuracy,simplicity.This approach could be used for clinical laboratory.

关 键 词：移液器 容量 比对与校准 放射性同位素法125碘标记 每分钟脉冲数计数 

分 类 号：R446[医药卫生—诊断学]