PBDE-47单独和与PCB153联合染毒对大鼠神经发育的影响  

Effects of PBDE-47 Single Exposed and Combined with PCB153 on Neurodevelopment in Rats

作　　者：何平[1] 王爱国[1] 夏涛[1] 黄南[1] 郭丽娟[1] 牛强[1] 陈学敏[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生系环境与健康教育部重点实验室,湖北武汉430030

出　　处：《环境与健康杂志》2008年第9期763-766,共4页Journal of Environment and Health

基　　金：国家自然科学基金重大项目(45090393);新世纪优秀人才支持计划(NCET)

摘　　要：目的探讨2,2’,4,4’-四溴联苯醚(2,2’,4,4’-tetrabromodiphenyl ethers,PBDE-47)单独和与2,2’,4,4’,5,5’六氯联苯(2.2’,4,4’,5,5’-hexachlorobiphenyl,PCB153)联合作用对 SD 大鼠学习记忆能力及海马 CA1区神经元超微结构的影响。方法将出生第10天清洁级 SD 大鼠随机分为1、5、10 mg/kg PBDE-47组、5 mg/kg PCB153组、1 mg/kg PBDE-47+5 mg/kg PCB153组、5 mg/kg PBDE-47+5 mg/kg PCB153组、10 mg/kg PBDE-47+5 mg/kg PCB153组和对照组(大豆油),每组18只,雌雄各半,按10 ml/kg 经口灌胃进行一次性染毒处理。在大鼠2个月龄时,进行 Morris 水迷宫实验和海马 CA1区神经元超微结构观察。结果低剂量组染毒大鼠(1 ms/ks PBDE-47组和1 mg/ks PBDE-47+5 mg/kg PCB153组)内质网和线粒体超微结构基本正常。随着染毒剂量的增加,内质网出现肿胀、扩张、脱颗粒,部分出现溶解、消失(5mg/kg PBDE-47组);线粒体出现肿胀、嵴断裂,严重者空泡化,进而出现神经元细胞浓缩(5 mg/kg PBDE-47+5 mg/kg PCB153组),甚至严重变性(10 mg/kg PBDE-47组和10 mg/kg PBDE-47+5mg/kg PCB153组)。PCB153和 PBDE-47联合染毒对大鼠水迷宫定位航行实验中找到平台的潜伏期和路径的影响存在交互作用(P<0.05),各染毒组的潜伏期和路径均长于对照组(P<0.05)。PCB153和 PBDE-47联合染毒对大鼠水迷宫空间探索实验中游经平台所在象限的路径(时间)与总路径(时间)的比值的影响存在交互作用(P<0.05):与对照组相比,所有染毒组的平台所在象限的路径(时间)与总路径(时间)的比值均下降(P<0.05)。超微病理及水迷宫实验结果均未发现性别差异。结论一定剂量的 PBDE-47对2月龄大鼠学习记忆能力及海马 CA1区神经元超微结构有损伤作用,PBDE-47与 PCB153在大鼠发育神经毒作用中存在交互作用。Objective To study the effects of 2, 2＇, 4, 4＇-tetrabromodiphenyl ethers （PBDE-47） single exposed and combined with 2, 2＇, 4, 4＇, 5, 5＇ -hexachtorobiphenyl （PCB153） on learning and memory ability and the uhrastructure of hippocampal CA1 region in rats. Methods Neonatal SD rats （CL grade） were randomly divided into groups, 9 male and 9 female in each, then were exposed to single PBDE-47 at doses of 1, 5, 10 mg/kg and combined with PCB153 at dose of 5 mg/kg, through gavage for one time. Soya oil was used as the solvent control. The learning and memory ability and uhrastructure of neurons in hippoeampal CA1 region were examined respectively 2 months after treatment. Results In the low dose groups （1 mg/kg PBDE-47 and 1 mg/kg PBDE-47 ＋ 5 mg/kg PCB153 treated group）, the uhrastructure of neurons in hippocampal CA1 region were as normal as that in the control group. As the dose increasing, in 5 mg/kg PBDE-47 group, the endoplasmic reticulum appeared swelling,expansion and degranulation. In 5 mg/kg PBDE-47 plus 5 mg/kg PCB153 group, the mitochondria appeared swelling, the ridge ruptured, the periplast puffed, and then the cytoplasm condensed. In the high dose groups （10 mg/kg PBDE-47 group and 10 mg/kg PBDE-47 plus 5 mg/kg PCB153 group）, the neurons showed acutely denatured, the periplast puffed, the cell organelle dissolved and the mitochondria vacuolizated. The interaction between PBDE-47 and PCB153 on the latency period and the general pathway was presented in place navigation test （P〈0.05）. Compared with the control, the latency period and general pathway were significantly increased in all treated groups （P〈0.05）. The interaction between PBDE-47 and PCB153 on the ratio [pathway （time） in platform quadrant/general pathway（time）] was present in spatial probe test（P〈0.05）. Compared with the control,the ratio [pathway（time） in platform quadrant/general pathway（time）] was notably decreased in all treated groups（P〈0.05）. No sex differences were found

关 键 词：2 2’ 4 4’-四溴联苯醚 2 2’ 4 4’ 5 5’六氯联苯 MORRIS水迷宫 超微结构 海马 

分 类 号：R994.6[医药卫生—毒理学]