毒死蜱和氯氰菊酯对小鼠细胞色素P450的影响  

Effect of Chlorpyrifos and Cypermethrin on Cytochrome P450 Activity in Mice

作　　者：崔永[1] 郭江峰[2] 徐步进[3] 陈子元[3] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]浙江理工大学生物工程研究所,浙江杭州310018 [3]浙江大学原子核农业科学研究所,浙江杭州310029

出　　处：《环境与健康杂志》2008年第9期789-792,共4页Journal of Environment and Health

基　　金：浙江省科技厅院士基金资助项目(J20030235)

摘　　要：目的研究毒死蜱和氯氰菊酯对小鼠细胞色素 P450(CYP)活力的影响。方法将30只8周龄清洁级 ICR 小鼠随机分为溶剂对照组(玉米油)、毒死蜱低(25.0 mg/kg)、高(50.0 mg/kg)剂量组和氯氰菊酯低(100.0 mg/kg)、高(200.0 mg/kg)剂量组,腹腔注射染毒,1次/d,连续3 d。染毒后,测定肝、肾和肺 CYP1A1、1A2、281、2E1和3A 的活力。结果无论染毒前、后,各组间小鼠体重比较,差异无统计学意义(P>0.05)。毒死蜱染毒组小鼠肺 CYP1A1,肾、肺 CYP2B1活力增高,氯氰菊酯染毒组肝、肾、肺 CYP1A1,肝、肺 CYP281,肝 CYP3A 活力增高,差异有统计学意义(P<0.05和 P<0.01)。毒死蜱对肝、肾CYP1A1,肾 CYP1A2,肝 CYP2E1,肝、肺 CYP3A 活力的影响,以及氯氰菊酯对肝、肺 CYP1A2,肾 CYP2B1,肝、肾、肺CYP2E1活力的影响具有性别特异性。毒死蜱对雌、雄鼠 CYP1A1、1A2、281、2E1,雄鼠 CYP3A 活力的影响。以及氯氰菊酯对雌、雄鼠 CYP1A2、2E1、3A,雌鼠 CYP2B1活力的影响具有器官特异性。结论毒死蜱和氯氰菊酯可增高小鼠部分器官CYP1A1和2B1的活力,氯氰菊酯还可增高部分器官 CYP3A 的活力。提示两种农药均可能有助癌性,并可能导致产生过量的活性氧而损伤 DNA。Objective To know the effect of pesticides chlorpyrifos and cypermethrin on activity of mouse cytochrome P450 （CYP）. Methods Thirty 8-week-old ICR mice （clean level） were randomly divided into the groups of vehicle control （com off）, the groups of chlorpyfifos at low dose （25.0 mg/kg） or high dose （50.0 mg/kg）, the groups of cypermethrin at low dose （100.0 mg/kg） or high dose （200.0 mg/kg）. The mice were administrated with the pesticides through intraperitoneal injection for 3 consecutive days, once a day. The mice were weighed before and after administration. After 3 days of treatment, the activity of CYP1A1, 1A2, 2B1, 2E1, 3A in liver, kidneys and lungs were determined. Results The difference of body weight among groups was not significant（P〉 0.05）. Chlorpyfifos increased the activity of CYP1A1 in lungs, CYP2B1 in kidneys and lungs. Cypermethrin increased activity of CYP1A1 in livers, kidneys and lungs, CYP2B1 in livers and lungs, CYP3A in livers. These increases were statistically significant （P〈0.05 and P〈0.01）. The effect of chlorpyrifos on activity of CYP1A1 in livers and kidneys, CYP1A2 in kidneys, CYP2E1 in livers, CYP3A in livers and lungs, and the effect of cypermethrin on activity of CYP1A2 in livers and lungs, CYP2B1 in kidneys, CYP2E1 in livers, kidneys and lungs were sex-specific. The effect of chlorpyrifos on activity of CYP1A1, 1A2, 2B1, 2El of females and males, CYP3A of males, and the effect of cypermethrin on activity of CYP1A2, 2E1, 3A of females and males, CYP2B1 of females were organ-specific. Conclusion Chlorpyrifos and cypermethrin can increase activity of CYP1A1 and 2B1 in some organs, and cypermethrin can also increase activity of CYP3A in some organs. It is suggested that both pesticides may have cocarcinogenicity, and they may result in an excess of reactive oxygen species （ROS） to damage DNA.

关 键 词：农药 细胞色素P450 毒死蜱 氯氰菊酯 

分 类 号：R994.6[医药卫生—毒理学]