陕西关中地区棉花黄萎菌营养亲和性研究  

Vegetative Compatibility of Verticillium Dahliae of Cotton in Guanzhong,Shaanxi

作　　者：许楠[1] 张慧霞[1] 杨家荣[1] 李艳[1] 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100

出　　处：《棉花学报》2008年第5期364-367,共4页Cotton Science

基　　金：欧盟科技合作项目(ICA4-CT-2001-10001)

摘　　要：由于陕西关中地区的泾阳菌系被定为致病性最强的生理Ⅰ型,本试验采用营养亲和性的方法对陕西关中地区采集的18个菌株以及陕西省植保所提供的新疆和田菌系、陕西泾阳菌系和河南安阳菌系进行了研究。经不同氮源利用的鉴定,结果为:21个菌株共获得364个nit突变株,其中nit1289个,占总量的79.40%;nitM72个,占总量的19.78%;nit3仅有3个。经营养体亲和性配对测试,21个菌株可分为3个营养亲合群(VCGs)。新疆和田菌系属于亲合群2(VCG2),河南安阳菌系属于亲合群3(VCG3)。其余的18个菌株属于亲和群1(VCG1)。B18没有产生突变体。且发现不同菌株营养体亲和产生的亲和带形态有差异,且具一定稳定性。由此可见,造成陕西关中地区棉花黄萎病发生严重并造成经济损失的主要原因是由于主要致病菌属于VCG1。Because Jingyang strain is the highest aggressive strain which comes from the area of Guanzhong, Shaanxi. We studies on vegetative compatibility tests for the 18 strains of Verticillium dahliae of Guanzhong, Shaanxi and the Hetian strain from Xinjiang, Jingyang strain, Anyang strain which supplied by the Institute of Plant Protection Shaanxi Academy of Agricultural Science. According to their phenotypes on the media containing only one of the three different nitrogen sources, respectively, the results are： there were about 364 mutants obtained from 21 strains induced on KPS media , in which 289 mutants were nit1, it accounted for 79. 40% of the total; 72 mutants were nitM, 19.78%; only three mutants were nit3. All except three strains, could be assigned to one vegetative compatibility group designated VCG1, the Hetian strain belongs to VCG2, the Anyang strain belongs to VCG3. B18 doesn＇t product the mutants. The morphological characters of vegetative compatibility zone among different compatible isolate pairs were different and stable. In a word, the main reason for Verticillium dahliae of the strains of Guanzhong leading to seriously lost on the economy is that their dominant population belong to the VCG1.

关 键 词：营养体亲和性 NIT突变体 棉花黄萎病菌 陕西关中 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]