N型钙通道在大鼠福尔马林内脏炎症痛中的作用研究  

THE ROLE OF N-TYPE CALCIUM CHANNEL IN VISCERAL INFLAMMATORY PAIN INDUCED BY FORMALIN

作　　者：张颜波[1] 牛敬忠[1] 王轩[1] 杨明峰[1] 孙保亮[1] 

机构地区：[1]泰山医学院附属医院神经内科,泰安271000

出　　处：《中国疼痛医学杂志》2008年第4期230-232,共3页Chinese Journal of Pain Medicine

摘　　要：目的：观察N型钙通道在福尔马林诱导的内脏炎症痛中的作用。方法：选用成年健康Wistar大鼠，随机分5组：正常对照组（N）；单纯福尔马林直肠致炎组（F）；脊髓蛛网膜下腔插管组（IT），插管内注射生理盐水组（NaCl）；插管内注射SNX-111组（SNX-111）。记录实验组大鼠的疼痛行为反应，致痛后以15min为一个时间段，共记录8个时间段，计2h，分别计算疼痛分数；福尔马林致痛后30min用原子吸收光谱分析和La^3＋阻断技术测定L6—S1-2阶段脊髓细胞内Ca^2＋含量。结果：F、IT组与NaCl组8个时间段内疼痛分数无显著性差异（P〉0．05）；IT组与F组相比也无明显差异（P〉0．05）；SNX-111组与NaCl组在致痛后75min内疼痛分数明显减少（P〈0．01或P〈0．05）。F、IT组与NaCl组在福尔马林致痛后30min脊髓细胞内Ca^2＋含量无明显变化（P〉0．05）；IT组与F组相比也无明显差异（P〉0．05）；SNX-111组与NaCl组相比Ca^2＋含量明显减少（P〈0．01）。结论：SNX-111能明显减轻福尔马林内脏炎症痛行为反应，降低脊髓内Ca^2＋含量，提示N型钙通道可能在福尔马林致内脏炎症痛感受中起重要作用。Objective： To examine the role of N-type calcium channel in visceral inflammatory pain induced by formalin. Methods： Adult healthy Wistar rats were divided into 5 groups randomly： normal group （N） ; group F： only injected of formalin in the submueosa of rectum; group IT： intrathecal insertion of a PE-10 tube ; group NaCl ： intrathecal injection of 0.9% NaCl ; group SNX-111 ： intrathecal injection of SNX-111. The numerical score for pain behaviors was recorded every 15 minutes for 2 hours. Pain scores were counted respectively. The intracellular calcium contents of L6 - S1 -2 segments of the spinal at 30 min time point after formalin injection were examined by the technique of atomic absorption spectectomy with La^3 ＋ blockage. Results ： There was no significant diffference of pain scores in 8 time points between group F or IT and group NaCl （ P 〉 0.05 ） ; and between group IT and group F （P 〉 0.05 ） ; pain scores of group SNX-111 was decreased significantly in the former 75 minutes than that of group NaCl（P 〈 0.05 or P 〈 0.01 ）. There was no significant difference of the intracellular calcium contents at 30 min time point after formalin injection between group F or IT and group NaCI（ P 〉0.05 ） ;and between group IT and group F（ P 〉0.05 ） ; the intracellular calcium contents of group SNX-111 were decreased significantly than that of group NaCl （ P 〈 0.01 ）. Conclusion ： SNX-111 can significantly alleviate the reaction of visceral inflammation induced by formalin and decrease the intracellular calcium contents. Which indicates that N-type calcium channel may play an important role in nociception of visceral inflammation induced by formalin.

关 键 词：N型钙离子通道 SNX-111 福尔马林 内脏 疼痛 

分 类 号：R402[医药卫生—临床医学]