Vip3A基因植物表达载体的构建及其对烟草的遗传转化  

Construction of Plant Expression Vectors of Vip3A Gene and Transformation in Tobacco

作　　者：马作江[1] 邓伟[1] 杨迎伍[1] 李正国[1] 

机构地区：[1]重庆大学生物工程学院基因工程研究中心重庆市高校功能基因与调控新技术重点实验室,重庆400044

出　　处：《生物技术通报》2008年第5期108-111,共4页Biotechnology Bulletin

基　　金：国家自然科学基金项目(3471214);重庆市科委自然基金重点项目(2007BA1005)

摘　　要：为了研究Vip3A基因在转基因抗虫植物中的应用,利用PCR技术克隆了苏云金芽孢杆菌的Vip3A基因和烟草的EF1α启动子,以pBI121质粒为基本载体,构建了分别由组成型CaMV35S启动子和花特异表达的EF1α启动子驱动Vip3A基因的植物表达载体pBIVip3A和pBIEFVip3A,并通过农杆菌介导的方法对烟草进行了遗传转化。经PCR检测,外源基因已整合到烟草基因组中。In order to study the application of Vip3A gene in transgenic insect-resistant plants,Vip3A gene of Baciius thuringiensis and EF1 α promoter of tobacco were cloned by PCR. Two plant expression vectors pBIVip3A and pBIEFVip3A were constructed,in which the Vip3A gene was driven by the constitutive promoter CaMV35S and petal tissue-specific promoter EF1α respectively. Tobaccos were tansformed by Agrobacterium containing the constructed vectors. The transgenic lines were selected by PCR detecstion.

关 键 词：VIP3A基因 EF1α启动子 载体构建 苏云金芽孢杆菌 烟草 

分 类 号：Q943.2[生物学—植物学] S476.11[农业科学—农业昆虫与害虫防治]