大鼠急性甲醇中毒视神经损伤实验研究  

Experimental study on optic nerve injury in rats with acute methanol intoxication

作　　者：童宗武[1] 陈晋[1] 杨崇猛[1] 高琨[1] 苏少明[1] 邱明[1] 高秀芳[1] 孙阳[1] 

机构地区：[1]云南省玉溪市人民医院肾内科,玉溪653100

出　　处：《中国血液净化》2008年第9期485-487,共3页Chinese Journal of Blood Purification

基　　金：课题获云南省玉溪市科技进步三等奖

摘　　要：目的探讨大鼠急性甲醇中毒视神经损伤时间窗及程度。方法用SD大鼠经口灌注甲醇,灌注后分不同时段处死大鼠取血测甲醇浓度、取视神经做病理检查。结果LD50从2h开始出现较为明显的损伤,4h后程度最重。LD0损伤最重的时间段在8h后。不论是LD0、LD50在损伤程度达到最严重之后,连续观察24h、48h、72h随着血液中甲醇浓度的降低,视神经的损伤仍然明显地持续存在。结论急性甲醇中毒对视神经早期(0.5h)就有损伤,损伤程度和剂量浓度有关,视神经的损伤最严重出现在血液中甲醇浓度高峰值的4h后,随时间推移甲醇在大鼠体内的继续弥散,致使损害迁延。Objective To study the time course and degree of optic nerve injury in rats with acute methanol intoxication. Methods Sprague-Dawley rats were fed methanol orally and sacrificed at different point of time after the gavage. Blood samples were taken for methanol concentration measurement, and optic nerves were removed for pathological examination. Results In rats taken methanol to the dose of LD50, significant optic nerve injury began after 2 hours. The injury progressed along with time and reached the peak after 4 hours. In rats taken methanol to the dose of LD0, optic nerve injury reached the peak after 8 hours. In both groups of rats, optic nerve injury persisted even though blood methanol concentration became lower after 24h, 48h and 72h of methanol administration. Conclusion Optic nerve injury occurs as early as half an hour after acute methanol intoxication. The injury degree is related to the dose and blood concentration of methanol. Optic nerve injury reaches the peak after the presence of the highest blood methanol concentration for 4 hours. Optic nerve injury persists even though blood methanol concentration goes down after the methanol administration.

关 键 词：大鼠 急性甲醇中毒 视神经损伤 

分 类 号：R459.5[医药卫生—治疗学] R99[医药卫生—临床医学]