高温胁迫下外源钙对菊花叶片光合机构与活性氧清除酶系统的影响  

Effects of exogenous Ca^(2+)on leaf photosynthetic apparatus and active oxygen scavenging enzyme system of chrysanthemum under high temperature stress

作　　者：孙宪芝[1] 郭先锋[2] 郑成淑[1] 王文莉[1] 梁芳[1] 

机构地区：[1]山东农业大学园艺科学与工程学院作物生物学国家重点实验室,山东泰安271018 [2]山东农业大学林学院,山东泰安271018

出　　处：《应用生态学报》2008年第9期1983-1988,共6页Chinese Journal of Applied Ecology

基　　金：教育部留学回国人员项目(33206);国家"十一五"科技支撑计划资助项目(2006BAD10B07)

摘　　要：以切花菊品种‘神马’为材料,研究了高温胁迫下外源Ca2+对菊花光合系统及活性氧清除酶系统的影响,探讨了Ca2+可能的作用机制.结果表明:高温胁迫下外源Ca2+抑制了净光合速率(Pn)与实际量子效率(ΦPSⅡ)的大幅度降低,24h后,二者分别比对照高出31.11%与21.88%,而初始荧光(Fo)比对照降低了13.19%;Ca2+明显激活了高温胁迫下叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,活性氧得到及时清除,24h后,膜脂过氧化产物丙二醛(MDA)的积累量与相对电导率(REC)分别比对照降低29.20%与35.81%,缓解了短期高温胁迫对菊花光合系统的破坏.Taking cut flower chrysanthemum ‘Jinba’ as test material, the effects of exogenous Ca2 ＋ on its photosynthetic system and antioxidant enzyme activities under high temperature stress were investigated, with the possible action mechanisms of Ca2＋ discussed. The results showed that under high temperature stress, Ca2＋ addition greatly inhibited the net photosynthesis rate （Pn） and quantum yield of PS Ⅱ electron transport （ qbpsn ）. After 24 h treatment, the P and ΦpsⅡ were increased by 31.11% and 21.88% , respectively, and the initial fluorescence （Fo） decreased by 13.19%, compare with the control. Ca2. addition also greatly enhanced the activities of SOD, POD and CAT, and thus, the active oxygen was scavenged timely. After 24 h treatment, the MDA accumulation and REC were 29.20% and 35.81% lower than the control, respectively. In conclusion, Ca2＋ addition could efficiently protect chrysanthemum leaves from the damage in photosynthetic apparatus under short-term high temperature stress.

关 键 词：菊花 高温胁迫 CA2+ 光合机构 抗氧化酶 

分 类 号：S682.11[农业科学—观赏园艺]