氯化钠胁迫对南瓜根系游离态多胺含量和活性氧水平的影响  

Effects of NaCl stress on free polyamines content and reactive oxygen species level in pumpkin roots

作　　者：周俊国[1] 扈惠灵[2] 朱月林[1] 张古文[1] 刘正鲁[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]河南科技学院园艺系,河南新乡453003

出　　处：《应用生态学报》2008年第9期1989-1994,共6页Chinese Journal of Applied Ecology

基　　金：河南省重点科技攻关计划资助项目(072102120006)

摘　　要：以中国南瓜杂交种'360-3×112-2'和黑籽南瓜为试验材料,在营养液栽培条件下研究了NaCl胁迫对两种南瓜植株生长、根系活性氧水平和游离态多胺含量的影响.结果表明,NaCl胁迫10d后,与对照相比,两种南瓜植株生长都受到明显抑制,但中国南瓜杂交种比黑籽南瓜植株的耐盐性强.NaCl胁迫使南瓜根系O2.-产生速率和H2O2含量提高,且黑籽南瓜的O2.-产生速率和H2O2含量高于中国南瓜杂交种.两种南瓜根系中腐胺(Put)、亚精胺(Spd)、精胺(Spm)和多胺(PAs)含量及Put/PAs高于对照,并呈现先升后降的趋势;根系中(Spd+Spm)/Put低于对照,呈现先降后升的趋势.中国南瓜杂交种根系中Put含量和Put/PAs低于黑籽南瓜,而Spd、Spm含量和(Spd+Spm)/Put高于黑籽南瓜.表明两种南瓜根系中多胺含量的升高对减少或清除组织中的活性氧有积极作用,Put向Spd、Spm的转化有利于增强植株的耐盐性;中国南瓜杂交种'360-3×112-2'的耐盐性高于黑籽南瓜与其根系中Put/PAs较低、(Spd+Spm)/Put和PAs含量较高,使其清除活性氧能力较强有关.Taking Cucurbita moschata Duch hybrid 360-3 × 112-2 and C. ficifolia Bouche as test materials, the effects of NaC1 stress on their plant growth and the O2^- production rate and H202 and free polyamines （PAs） contents in their roots were studied with hydroponic culture. The results showed that after 10 d NaC1 stress, the plant growth of the two pumpkin varieties was strongly inhibited, compared with the control, and C. ficifolia was more injured than hybrid 360-3 × 112-2. Un- der NaC1 stress, the root O2 - production rate and n202 content of the two pumpkin varieties were increased, but their absolute values were lower in hybrid 360-3 ×112-2 than in C. ficifolia. The contents of PAs, putrescine （ Put）, spermidine （Spd） and spermine （Spm） and Put/PAs ratio in the roots of the two pumpkin varieties were always higher than the control and had a trend of increased first and decreased then ; while the （ Spd ＋ Spm）/Put ratio was lower than the control and decreased first and increased then. Compared with C. ficifolia Bouche, hybrid 360-3 × 112-2 always had a lower Put/PAs ratio and a lower Put content in its roots, but the （Spd ＋ Spm）/Put ratio and Spd and Spm contents were always higher. It was concluded that under NaC1 stress, the increasing PAs content in the roots of test materials played an active role in decreasing or scavenging reactive oxygen species （ROS）. The conversion of Put to Spd and Spm was advantageous to the increase of plant salt tolerance. The higher salt tolerance of hybrid 360-3 ×112-2 was closely related to the lower Put/PAs ratio and the higher （Spd ＋ Spm）/Put ratio and PAs content in its roots, and thus, the stronger capacity to scavenge ROS.

关 键 词：NACL胁迫 中国南瓜杂交种 黑籽南瓜 游离态多胺 活性氧 

分 类 号：S642.1[农业科学—蔬菜学]