草甘膦对大豆超微结构及光合指标影响的研究  

Soybean ultrastructure and photosynthesis index as affected by glyphosate

作　　者：卜贵军[1] 刘洪梅[1] 李英[1] 崔琳[1] 王学东[1] 

机构地区：[1]东北农业大学生命中心,黑龙江哈尔滨150030

出　　处：《电子显微学报》2008年第4期322-330,共9页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.30471093)~~

摘　　要：利用透射电镜和扫描电镜,研究转基因抗草甘膦大豆和非抗草甘膦大豆东农42喷施草甘膦后大豆光合器官叶片的超微结构变化,并结合叶绿素含量、光合速率的测定,对比分析了抗草甘膦大豆和东农42在结构及生理功能上对草甘膦的表现及结构与功能变化的关系。研究结果表明抗草甘膦大豆对草甘膦的胁迫可以看作是一种适应。它首先经过一个损伤期,然后进入修复期,最终到基本恢复,表现出对草甘膦的抗性。而东农42的光合系统及光合功能则被草甘膦所彻底破坏。本文对其原因进行探究,以期为转基因作物育种尤其是超微结构方面的研究及草甘膦作用机理的研究有一定帮助。By using transmission electron microscope （TEM） and scanning electron microscope （SEM）, we studied ultrastructural changes in photosynthesis organ-leaves of glyphosate-resistant soybean and soybean Dongnong42, in response to glyphosate. Besides, through the measurement of chlorophyll content, photosynthetic rate as affected by glyphosate, we analyzed glyphosate-resistant soybeans and soybean Dongnong 42 in the physiological functions and ultrastructure and the relationship between them as affected by glyphosate. The results showed that glyphosate-resistant soybean was affected by glyphosate stress. After living through an injury period, it stepped into a repair period, which ultimately showed resistance to glyphosate. But soybean Dongnong 42 photosynthetic system and function were thoroughly destroyed by glyphosate. In addition, we did our best to probe the reasons. It might be useful in the field of transgenic crops breeding, particularly in the uhrastructural studies and plays an important role in glyphosate mechanism study in the future.

关 键 词：抗草甘膦大豆 超微结构 光合作用 嗜锇颗粒 

分 类 号：S565.1[农业科学—作物学] Q336[生物学—遗传学]