百合鳞茎苯丙氨酸解氨酶提取条件的优化  

Optimization on Extractive Technique of Phenylalanine Ammonia-lyase in Lily Bulb

作　　者：孙红梅[1] 赵爽[1] 王春夏[1] 陈丽静[1] 王锦霞[1] 

机构地区：[1]沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,沈阳110161

出　　处：《植物研究》2008年第5期594-598,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金(30400307);辽宁省教育厅基金(2004F084,20060787)

摘　　要：研究兰州百合(Lilium davidiivar.unicolor)鳞茎内PAL的最佳提取条件结果表明:pH8.8的硼砂缓冲液为最适缓冲液;百合鳞茎的PAL不耐酸碱,更不耐酸;最适反应温度为40℃;随着水浴时间延长和水浴温度提高,PAL活性逐渐降低,但40℃水浴30 min后,酶活性仍保留47%;最适底物浓度为0.032 mol.L-1;磨样时加入PVP 0.5 g,能够明显提高PAL活性。Optimum extractive technique of phenylalanine ammonia-lyase （PAL） in the bulb of Lilium davidii var. unicolor was studied. The results indicated that Borax solution with pH8.8 was the best buffer. PAL in lily bulb was not able to endure the strong acid and alkali, especially the former. The PAL activity declined gradually along with the increase of temperature and prolonging of bath duration, but the activity still remained 47% after 30 min in 40℃, and it was the optimum bath temperature. O. 032 mol·L^-2 substrate was the best, and grinding with 0.5 g PVP could improve the PAL activity obviously.

关 键 词：兰州百合 鳞茎 苯丙氨酸解氨酶 提取技术 

分 类 号：S644.1[农业科学—蔬菜学] Q945.78[农业科学—园艺学]