梅毒患者外周血树突细胞亚型及其表达不同细胞因子的检测  

Detection of peripheral dendritic cell subsets and T cell subpopulations expressing different intracellular cytokines in patients with syphilis

作　　者：郭义龙 许敏鸿 蔡小丹 杨启平 

机构地区：[1]广东省汕头市皮肤性病防治院,515041

出　　处：《中华皮肤科杂志》2008年第9期612-615,共4页Chinese Journal of Dermatology

基　　金：广东省汕头市重点科技计划资助项目（2005116）

摘　　要：目的探讨梅毒患者外周血树突细胞（DC）各亚型及表达不同细胞因子T细胞亚群的频数在梅毒发病机制中的意义。方法流式细胞仪测定20例梅毒患者治疗前、治疗后和15例正常人外周血DC各亚型及表达不同细胞因子（包括IFN—γ、IL-2、IL-4、IL-10、IL-12）CD4^＋、CD8^＋ T细胞亚群的频数。结果20例梅毒患者外周血髓系DE亚型（CD11c^＋）的表达，治疗前、后比较有显著升高（t=3．339，P〈0．01），治疗前与对照组比较显著升高（P〈0．05）。梅毒患者外周淋巴系DC亚型（CD123^＋）的表达，治疗前、治疗后、对照组比较其差异均无统计学意义（P〉0．05）。梅毒患者外周血T细胞亚群胞质内细胞因子IFN-γ的表达，治疗前、后比较有显著升高（t=14．890，P〈0．01），治疗前与对照组比较显著升高（P〈0．01）；梅毒患者外周血T细胞亚群胞质内细胞因子IL-2、IL-4、IL—10、IL-12的表达，IL-2和IL～12治疗前、后比较有显著升高（t=10．899，4．292，P均〈0．01），治疗前与对照组比较显著升高（P〈0．05），IL-4和IL-10治疗前与对照组比较显著降低（P〈0．01）。梅毒患者外周血CD11c^＋、CD123^＋的表达与IFN-γ、IL-12的百分比不存在相关关系（P均〉0．05）。结论IFN-γ、IL-2和IL-12可能在梅毒清除过程中发挥免疫调节作用，而IL-4、IL-10起免疫抑制作用。Objective To investigate the role of dendritic cell （DC） subsets and T cell subpopulations expressing different intracellular cytokines in the pathogenesis of syphilis. Methods Flow eytometry was utilized to measure the frequencies of peripheral DC subsets and IFN-γ-, IL-2-, IL-4-, IL-10-cxpressing peripheral CD4^＋ and CD8^＋ T cell subpopulations in 20 patients with syphilis before and after therapy, and in 15 healthy human controls. Re.malts The frequency of CD11c^＋ DC was significantly lower in pretreated patients than that in treated patients （P 〈 0.01 ）, but higher than that in the normal controls （P 〈 0.05 ）, and the same was true for the frequency of IFN-γ-expressing T cells, IL-2-expressing T cells, and IL-12-expressing T cells. By contrast, a decrease was noticed in the frequency of IL-4-expressing T cells and IL-10-expressing T cells in pretreated patients compared with that in the normal controls. Furthermore, in patients with syphilis, the frequency of CD11c^＋ and CD123^＋ DC was unrelated to the percentage of IFN-γ-expressing and IL-12- expressing T cells （both P 〉 0.05）. Conclusions IFN-γ, IL-2 and IL-12 may exert an immunoregulatory effect in the eliminating of Treponema pallidum, whereas IL-4 and IL-10 may perform an immanoinhibitory effect.

关 键 词：梅毒 树突细胞 T淋巴细胞 细胞因子类 

分 类 号：R759.1[医药卫生—皮肤病学与性病学] R392.12[医药卫生—临床医学]