生肌液复合封闭负压引流对创面肉芽组织增殖的作用  

Effect of Shengjiye combining vacuum-assisted closure on the proliferation of traumatic granulation tissues

作　　者：万明才[1] 冯峰[1] 王新卫[1] 郭建刚 朱太咏 黄满玉 毕军花 

机构地区：[1]河南省洛阳正骨医院骨髓炎科,河南省洛阳市471002 [2]河南省正骨研究院,河南省洛阳市471002

出　　处：《中国组织工程研究与临床康复》2008年第37期7250-7253,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:传统生肌中药促进创面愈合疗效独特,封闭负压引流技术是新兴的创面治疗方法。目的:从组织学角度观察生肌液和封闭负压引流技术相结合对实验动物创面愈合的影响。设计、时间及地点:随机对照动物实验,于2006-05/10在河南省正骨研究院生物工程实验室完成。材料:100只SD大鼠随机分为模型组、麻油纱布组、生肌液纱布组、封闭负压引流组、生肌液复合封闭负压引流组,每组20只。生肌液和麻油煎剂均由本院制剂室规范制备。方法:在每只大鼠背部制作2个皮肤切除伤创面模型。模型组等待创面自然愈合,麻油纱布组以两层麻油纱布敷于创面上,生肌液纱布组以两层生肌液纱布敷于创面上;封闭负压引流组给予创面封闭负压引流治疗。生肌液外用结合封闭负压引流组:给予创面封闭负压引流治疗,负压吸引间歇期从注药管注入生肌液。每组20只动物随机分4批各10个创面,第1,2,3个批次分别用于3,6,9天取材观察创面的愈合率和创面组织形态学变化。第4个批次观察创面愈合时间。主要观察指标:①创面的愈合率。②创面愈合时间。③创面组织形态学变化。结果:①各组创面第3天愈合率比较差异无显著性意义(P>0.05)。3d后封闭负压引流组及复合组愈合速度明显加快。第9天愈合率由高到低依次为复合组、封闭负压引流组、生肌液纱布组、麻油纱布组和模型组,组间比较差异有显著性意义(P<0.05)。各组愈合时间由快到慢排序同上。②第3天封闭负压引流组毛细血管数略高于复合组(P>0.05);第6天复合组毛细血管数高于封闭负压引流组(P<0.05);第3天各组成纤维细胞数均维持在较低水平,组间比较差异无显著性意义(P>0.05)第6天复合组成纤维细胞数高于封闭负压引流组(P<0.05)。结论:生肌液复合封闭负压引流技术可以更好地促进创面愈合。封闭负压引流技术在创面愈合�BACKGROUND： Traditional Chinese medicines have special effects on wound healing, and vacuum-assisted closure is a newly-applied method for wound healing. OBJECTIVE： To study effect of Shengjiye in combination with vacuum-assisted closure on wound healing regarding histological changes. DESIGN, TIME AND SETTING： A randomized control animal experiment was carried out at Laboratory of Bioengineering, Henan Orthopaedics Institute （Luoyang, Henan, China） from May to October in 2006. MATERIALS： One hundred SD rats were randomized into five groups： model group, sesame oil gauze group, Shengjiye gauze group vacuum-assisted closure group, and Shengjiye plus vacuum-assisted closure group. Each group had twenty animals. Shengjiye and sesame oil decoctum were prepared in the Henan Orthopaedics Institute （Luoyang, Henan, China）. METHODS： Two trauma models were induced by the excision of dorsal skin in each rat. The wounds of model group healed naturally, the wounds of the remaining groups were treated with sesame oil gauze, Shengjiye gauze, vacuum-assisted closure, and Shengjiye plus vacuum-assisted closure, respectively. Shengjiye was administrated during the intermission of vacuum aspiration. Ten wounds of each group were randomized into four patches. On days 3, 6 and 9, the former three patches were used for observing the healing rate and the histological changes, while the fourth patch for observing the healing time. MAIN OUTCOME MEASURES： The healing rate, healing time and histological changes of the wound. RESULTS： On day 3, there were no significant differences in the healing rate among different groups （P 〉 0.05）. The healing speed of the vacuum-assisted closure group and the Shengjiye plus vacuum-assisted closure group accelerated rapidly after three days. On day 9, the healing rate ranged from high to low, as Shengjiye plus vacuum-assisted closure group, vacuum-assisted closure group, Shengjiye gauze group, sesame oil gauze group, and model group. The differences among different groups

关 键 词：中药 封闭负压引流 创面愈合 组织构建 

分 类 号：R318[医药卫生—生物医学工程]