骨折并侧方液压脑损伤模型的建立  

Developing a rat model of fracture complicated by lateral fluid percussion brain injury

作　　者：罗同青[1] 李兵[1] 胡朝辉[1] 

机构地区：[1]广西医科大学第五附属医院柳州市人民医院,广西壮族自治区柳州市545001

出　　处：《中国组织工程研究与临床康复》2008年第37期7272-7276,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广西壮族自治区科技厅青年基金资助项目(桂科青0447055)~~

摘　　要：目的:拟建立一种伤情稳定、重复性好的骨折与脑损伤复合模型,为临床脑损伤骨折患者骨折愈合加速的机制研究提供动物模型。方法:实验于2007-04/08在广西医科大学动物实验中心完成。雄性SD大鼠72只,按体质量经SPSS15.0随机分为空白对照组(n=6),单纯骨折组(n=30),脑损伤骨折组(n=36)。用改良的脑损伤液压装置,采用侧方液压法建立SD大鼠脑损伤模型。用摆锯从胫骨外侧锯断胫骨中段,然后行克氏针髓腔逆行固定,制备胫骨骨折模型。结果:72只大鼠均进入结果分析。①伤后3h,脑损伤骨折组大鼠顶叶、额叶、枕叶蛛网膜下腔出血,大脑皮质及白质深部的基底节、海马、胼胝体、丘脑挫伤出血,苏木精-伊红染色显示,挫伤周围神经元变性、坏死。伤后1d挫伤灶周围神经元数量明显减少,可见血管周围出血及均质透明栓形成。②术后4周X射线平片显示脑损伤骨折组骨痂大于单纯骨折组且骨痂致密,骨痂直径及骨痂应力大于单纯骨折组(P<0.05)。结论:股骨离断及侧方液压制作的SD大鼠骨折脑损伤动物模型伤情稳定、重复性好,能满足脑损伤骨折实验研究的需要。AIM： To establish a model of traumatic brain injury complicated by bone fracture with stable injury and repeatability to provide animal models for mechanism studies of fracture healing in patients with traumatic brain injury. METHODS： The experiment was performed at the Animal Experimental Center of Guangxi Medical University from April to August 2007. Seventy-two male SD rats were randomized to blank control group （n = 6）, fracture group （n = 30）, and brain injury and fracture group （n = 36） according to body mass by SPSS 15.0. Using modified hydraulic equipment, the rat model of traumatic brain injury was established by fluid lateral percussion. Then middle tibia was sawn by pendular saw and reversely fixed by Kirschner wire to create tibia fracture models. RESULTS： Seventy-two rats were included in final analysis. ①Three hours after injury, hemorrhage was found in rat apical lobe, frontal lobe, end-lobe subarachnoid cavity, cerebral cortex, and basal ganglia of white matter, hippocampus, callositas, and cerebral ganglion of brain injury and fracture group. HE staining showed neuronal degeneration even necrosis surrounding traumatic sites. One day after injury, number of neurons around traumatic focus was significantly decreased, and hemorrhage surrounding vessels and even transparent thrombus were found. ②X-ray photograph 4 weeks postoperatively suggested that the size of bony callus in brain injury and fracture group was significantly larger and denser than fracture group. In addition, the diameter and the stiffness of callus was greater than B group （P 〈 0.05）. CONCLUSION： Femoral mutilation and fluid lateral percussion can made animal models of tibial fracture complicated by brain injury with stable injury and repeatability, which meet the demand of experimental studies.

关 键 词：胫骨骨折 创伤 液压 脑损伤 动物模型 组织构建 

分 类 号：R683[医药卫生—骨科学]