MMP-2、MMP-9在自体移植静脉的表达及活性变化  

A study on the expression and activity of MMP-2 and MMP-9 in autogenous vein grafts

作　　者：何伟[1] 刘志勇[1] 李旭东[1] 周建明[1] 王玉华[1] 

机构地区：[1]东南大学附属中大医院心胸外科,南京210009

出　　处：《江苏医药》2008年第9期921-924,共4页Jiangsu Medical Journal

摘　　要：目的探讨自体移植静脉基质金属蛋白酶(MMP)-2和MMP-9表达及活性的变化。方法建立兔自体静脉旁路移植模型后3、7、28d切取移植静脉,取对侧未移植静脉为自身对照。行HE和Verhoeff弹性纤维染色观察组织病理变化,计算机病理图像分析系统测量新生内膜厚度及面积,半定量逆转录-PCR检测静脉壁MMP-2和MMP-9mRNA表达,明胶酶谱法检测MMP-2、MMP-9活性,应用免疫组化法检测MMP-2蛋白表达。结果术后7、28d移植静脉内膜明显增生。与未移植静脉相比,术后3d,移植静脉MMP-2、MMP-9mRNA表达已明显增高,7d达峰值(P<0.01),术后28d渐下降至正常水平,但MMP-9mRNA表达仍维持较高水平。术后三个时间点移植静脉壁MMP-2、MMP-9活性显著增加。结论自体移植静脉MMP-2、MMP-9表达及活性显著增强,促进移植静脉内膜增生。Objective To investigate the expression and significance of matrix metalloproteinase （MM） P-2 and MMP-9 in autogenous vein grafts. Methods Autogenous vein grafts model was established in 24 rabbits. Vein samples were harvested on the 3rd, 7th and 28th day after operation. Intimal areas were measured and intimal thickness was calculated. Expressions of MMP-2 mRNA and MMP-9 mRNA in vein grafts were detected by reverse transcription-PCR. Substrate gel zymography was used to determine the proteolytic activity. Results Intimal thickness was significantly increased on the 7th and 28th postoperative day. The expressions of MMP-2 and MMP-9 mRNA enhanced rapidly on the 3rd day and peaked on the 7th day （P〈0. 01）. Substrate gel zymography demonstrated that the proteolytic activity of MMP-2 and MMP-9 was significantly increased after surgery. Conclusion The expression and activity of MMP-2 and MMP-9 rise in autogenous vein grafts on the early days after operation, which may facilitate the intimal hyperplasia.

关 键 词：内膜增生 静脉移植 基质金属蛋白酶 

分 类 号：R456[医药卫生—治疗学]