宫内感染致脑损伤新生鼠血清及脑组织S-100含量的变化  

An increase of S-100 protein in serum and brain tissues of neonatal rats with brain injury caused by intrauterine infection

作　　者：李红英[1] 李晓捷[2] 姜志梅[2] 

机构地区：[1]南京医科大学附属南京儿童医院康复科,210008 [2]佳木斯大学康复医学院

出　　处：《江苏医药》2008年第9期934-935,F0003,共3页Jiangsu Medical Journal

摘　　要：目的探讨S-100能否作为脑损伤早期诊断生化指标。方法孕第18、19天的孕鼠连续两天腹腔注射脂多糖(LPS)500μg.kg-1.d-1制备脑损伤动物模型。取脑组织皮质、海马、内囊、胼胝体进行电镜观察;采用酶联免疫法测定血清和脑组织中S-100含量。结果实验组仔鼠脑组织有明显的损伤改变,血清及脑组织中S-100含量明显高于对照组(P<0.01)。结论S-100可以作为脑损伤早期诊断的指标。Objective To study whether serum level of S-100 can become a marker of early diagnosis in brain injury caused by intrauterine infection. Methods The rat model of brain injury was developed by consecutively injecting LPS （500μg/kg） for two days in pregnant rats of gestation day 18 and 19. The changes of uhrastructure in the internal capsule, hippocampal, cortex and callus were observed. The levels of S-100 in serum and brain was detected by ELISA method. Results The obvious damage was seen in the brain of neonatal rats in experimental group with a significant increase of S-100 in serum and brain compared with that in control group （P〈0. 01）. Conclusion Serum level of S-100 can be taken as a marker for early diagnosis of brain injury.

关 键 词：宫内感染 脑损伤 S-100 

分 类 号：R722[医药卫生—儿科]