光周期和高脂食物对布氏田鼠能量代谢和产热的影响  

Effects of photoperiod and high fat diet on energy intake and thermogenesis in Brandt's voles Lasiopodomys brandtii

作　　者：赵志军[1,2] 陈竞峰[1] 王德华[1] 

机构地区：[1]中国科学院动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京100080 [2]聊城大学农学院

出　　处：《动物学报》2008年第4期576-589,共14页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金委(No.30625009;No.30430140);科技部973项目(No.2007BC109103);中国科学院(No.KSCX2-YW-N-06)的资助~~

摘　　要：为了研究光周期和高脂食物对小型哺乳动物能量代谢和产热的影响,将成年雌性布氏田鼠(Lasiopodomys brandtii)分别驯化于长光照低脂、高脂食物和短光照低脂、高脂食物,7周后测定动物的体重、能量摄入、产热、血清瘦素浓度以及褐色脂肪组织解偶联蛋白1(BAT-UCP1)含量等参数。结果发现:1)短光照抑制体重增长、降低体脂重量和血清瘦素水平,增加非颤抖性产热(NST)和UCP1含量;2)高脂食物使摄入能减少和消化率提高,但未显著影响体重、基础代谢率、NST、UCP1含量和血清瘦素;3)血清瘦素与摄入能不相关,但与体脂含量正相关。结果暗示:短光照下瘦素作用敏感性增加和产热能力增强,可能介导了抵抗高脂食物诱导的肥胖。在野外条件下草食性的布氏田鼠能通过能量代谢和产热的适应性调节避免体重的过度增长,有利于降低捕食风险,增强生存能力。同时布氏田鼠是研究食物诱导肥胖机理的一个好模型。To investigate the roles of photoperiod and high fat diet in the regulation of energy budget and thermogenesis, adult female Brandt＇s voles Lasiopodomys brandtii were acclimatized to one of 4 regimens, 1 ） long day and low fat diet, LL; 2） long day and high fat diet, LH; 3） short day and low fat diet, SL; 4） short day and high fat diet, SH. After a 7-week acclimation period, we determined body mass, energy, budget, basal metabolic rate （BMR） and nonshivering thermogenesis （NST）, digestive tract morphology, serum leptin level, cytochrome e oxidase （COX） activity and uncoupling protein 1 （UCP1） content in brown adipose tissue （BAT）. The results showed that short days induced decreases in body mass, body fat mass and serum leptin level, and increases in NST, COX activity and UCP1 content. There were no significant effects of diets varying in fat content on body mass, BMR, NST, UCP1 content and serum leptin levels. However, high-fat diet significantly reduced energy intake, mass with content and wet mass of the total digestive tract, and elevated apparent digestibility. Serum leptin levels were positively correlated with body fat mass, however, not with energy intake. Our results suggest that Brandt＇s voles resist high-fat diet induced obesity, which might be mediated by the increased sensitivity of leptin and enhanced thermogenesis during short photoperiods. Brandt＇s voles, a strict herbivore in the field, can prevent excessive obesity by adaptive regulation of energy metabolism and thermogenesis. Also Brandt＇s vole is a potential animal model for studying dietary-induced obesity

关 键 词：布氏田鼠 光周期 高脂食物 体重 能量平衡 产热 瘦素 解偶联蛋白-1 

分 类 号：Q4[生物学—生理学]