基于CATS法的江豚SNPs位点筛选  

Single nucleotide polymorphisms of the finless porpoise Neophocaena phocaenoides revealed by using comparative anchor tagged sequences

作　　者：李树珍[1] 万慧荣[1] 杨光[1] 

机构地区：[1]南京师范大学生命科学学院江苏省生物多样性与生物技术重点实验室,南京210097

出　　处：《动物学报》2008年第4期719-724,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(No.30770294);教育部新世纪优秀人才支持计划(No.NCET-07-0445);教育部博士点基金(No.20060319002);江苏省高校重大基础研究计划支持项目(No.07KJA18016)~~

摘　　要：通过比较锚定序列示踪(Comparative Anchor Tagged Sequences,CATS)法,选择人、小鼠及其它哺乳动物中已知的5对CATS引物,通过PCR反应从江豚(Neophocaena phocaenoides)基因组中扩增出相应的DNA片段,结合变性高效液相色谱(Denaturing High Performance Liquid Chromatography,DHPLC)和DNA直接测序等技术,进行江豚单核苷酸多态性(Single nucleotide polymorphisms,SNPs)位点的筛选。通过不同个体同源序列的比对分析,发现其中的4个DNA片段具有多态性,共检测出7个SNPs位点,即大约每370bp出现1个SNPs。本研究为进一步利用CATS法获得江豚及其它野生动物种群的SNPs位点并进行种群遗传学分析奠定了基础。Comparative Anchor Tagged Sequences （CATS） were used to screen novel single nucleotide polymorphisms （SNPs） in the finless porpoise Neophocaena phocaenoides. Five loci were screened by using Polymerase Chain Reaction （PCR）, Denaturing High Performance Liquid Chromatography （DHPLC）, and direct sequencing. All the primer sets were based upon gene sequences from human, mouse and some other mammals. Seven SNPs were identified from four of the five sequenced regions, yielding a SNP on average every 370 base pairs （bp）. This study may provide a reliable basis for acquiring more SNPs through the CATS method in the finless porpoise and other wild animals

关 键 词：江豚 SNPS CATS DHPLC 

分 类 号：Q953[生物学—动物学]